Reference Report

Reference
Citation	McCartney, B.M., Dierick, H.A., Kirkpatrick, C., Moline, M.M., Baas, A., Peifer, M., Bejsovec, A. (1999). Drosophila APC2 is a cytoskeletally-associated protein that regulates wingless signaling in the embryonic epidermis. J. Cell Biol. 146(6): 1303--1318. (Export to RIS)
FlyBase ID	FBrf0111426
Publication Type	Research paper
PubMed ID	10491393
PubMed Abstract	The tumor suppressor adenomatous polyposis coli (APC) negatively regulates Wingless (Wg)/Wnt signal transduction by helping target the Wnt effector beta-catenin or its Drosophila homologue Armadillo (Arm) for destruction. In cultured mammalian cells, APC localizes to the cell cortex near the ends of microtubules. Drosophila APC (dAPC) negatively regulates Arm signaling, but only in a limited set of tissues. We describe a second fly APC, dAPC2, which binds Arm and is expressed in a broad spectrum of tissues. dAPC2's subcellular localization revealed colocalization with actin in many but not all cellular contexts, and also suggested a possible interaction with astral microtubules. For example, dAPC2 has a striking asymmetric distribution in neuroblasts, and dAPC2 colocalizes with assembling actin filaments at the base of developing larval denticles. We identified a dAPC2 mutation, revealing that dAPC2 is a negative regulator of Wg signaling in the embryonic epidermis. This allele acts genetically downstream of wg, and upstream of arm, dTCF, and, surprisingly, dishevelled. We discuss the implications of our results for Wg signaling, and suggest a role for dAPC2 as a mediator of Wg effects on the cytoskeleton. We also speculate on more general roles that APCs may play in cytoskeletal dynamics.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (3)
	Df(3R)crb87-4 Df(3R)crb87-5 Df(3R)crb-F89-4
Alleles (8)
	Apc2^ΔS arm⁸ dsh⁷⁵ pan³ Scer\GAL4^e22c sgg^unspecified wg^l-17 wg^Scer\UAS.cHa
Constructs (1)
	P{UAS-wg.H}
Genes (16)
	Apc Apc2 arm BicD dsh en pan pros Scer\GAL4 sgg shg wg βTub56D βTub60D βTub85D βTub97EF
Insertions (1)
	P{en2.4-GAL4}e22c