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Citation
Spradling, A.C., Stern, D., Beaton, A., Rhem, E.J., Laverty, T., Mozden, N., Misra, S., Rubin, G.M. (1999). The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes.  Genetics 153(1): 135--177.
FlyBase ID
FBrf0111489
Publication Type
Research paper
Abstract
A fundamental goal of genetics and functional genomics is to identify and mutate every gene in model organisms such as Drosophila melanogaster. The Berkeley Drosophila Genome Project (BDGP) gene disruption project generates single P-element insertion strains that each mutate unique genomic open reading frames. Such strains strongly facilitate further genetic and molecular studies of the disrupted loci, but it has remained unclear if P elements can be used to mutate all Drosophila genes. We now report that the primary collection has grown to contain 1045 strains that disrupt more than 25% of the estimated 3600 Drosophila genes that are essential for adult viability. Of these P insertions, 67% have been verified by genetic tests to cause the associated recessive mutant phenotypes, and the validity of most of the remaining lines is predicted on statistical grounds. Sequences flanking >920 insertions have been determined to exactly position them in the genome and to identify 376 potentially affected transcripts from collections of EST sequences. Strains in the BDGP collection are available from the Bloomington Stock Center and have already assisted the research community in characterizing >250 Drosophila genes. The likely identity of 131 additional genes in the collection is reported here. Our results show that Drosophila genes have a wide range of sensitivity to inactivation by P elements, and provide a rationale for greatly expanding the BDGP primary collection based entirely on insertion site sequencing. We predict that this approach can bring >85% of all Drosophila open reading frames under experimental control.
PubMed ID
PubMed Central ID
PMC1460730 (PMC) (EuropePMC)
DOI
Related Publication(s)
Review
Genomics. The millennium flies in.
Burtis and Hawley, 1999, Nature 401(6749): 125, 127 [FBrf0111336]
Erratum
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes.
Spradling et al., 1999, Genetics 153(3): 1491 [FBrf0125013]
Personal communication to FlyBase
Alleles of the lines in the P-element paper.
Beaton, 1999.12.12, Alleles of the lines in the P-element paper. [FBrf0125032]
Helping FlyBase with P-element paper.
Spradling, 1999.12.9, Helping FlyBase with P-element paper. [FBrf0125024]
Associated Information
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Aberrations (161)
    Alleles (1351)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Genes (979)
    Datasets (3)
    Natural transposons (2)
    Insertions (4153)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Transgenic Constructs (2)