Reference Report

Reference
Citation	Deng, W., Leaper, K., Bownes, M. (1999). A targeted gene silencing technique shows that Drosophila myosin VI is required for egg chamber and imaginal disc morphogenesis. J. Cell Sci. 112(21): 3677--3690. (Export to RIS)
FlyBase ID	FBrf0111848
Publication Type	Research paper
PubMed ID	10523504
PubMed Abstract	We report that Drosophila unconventional myosin VI, encoded by Myosin heavy chain at 95F (Mhc95F), is required for both imaginal disc and egg chamber morphogenesis. During oogenesis, Mhc95F is expressed in migrating follicle cells, including the border cells, which migrate between the nurse cells to lie at the anterior of the oocyte; the columnar cells that migrate over the oocyte; the centripetal cells that migrate between the oocyte and nurse cells; and the dorsal-anterior follicle cells, which migrate to secrete the chorionic appendages. Its function during development has been studied using a targeted gene silencing technique, combining the Gal4-UAS targeted expression system and the antisense RNA technique. Antibody staining shows that the expression of myosin 95F is greatly decreased in follicle cells when antisense Mhc95F RNA is expressed. Interfering with expression of Drosophila myosin VI at various developmental stages frequently results in lethality. During metamorphosis it results in adult flies with malformed legs and wings, indicating that myosin VI is essential for imaginal disc morphogenesis. During oogenesis, abnormal follicle cell shapes and aberrant follicle cell migrations are observed when antisense Mhc95F is expressed in follicle cells during stages 9 to 10, suggesting that the Drosophila myosin VI is required for follicle cell epithelial morphogenesis.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Alleles (18)
	Ecol\lacZ^Scer\UAS.cUa jar^a.Scer\UAS jar^C865 jar^Scer\UAS.cDa Scer\GAL4^bves-C648 Scer\GAL4^C22c Scer\GAL4^c532 Scer\GAL4^C535 Scer\GAL4^C577 Scer\GAL4^C601a Scer\GAL4^C612b Scer\GAL4^C673 Scer\GAL4^C714 Scer\GAL4^C726b Scer\GAL4^C789 Scer\GAL4^C844 Scer\GAL4^fng-c606 Scer\GAL4^jar-C865
Constructs (4)
	P{GAL4} P{UAS-jar.a} P{UAS-jar.D} P{UAS-lacZ.U}
Genes (5)
	Ecol\lacZ jar Scer\GAL4 zip α-Spec
Insertions (14)
	P{GAL4}C22c P{GAL4}C535 P{GAL4}C577 P{GAL4}C601a P{GAL4}C612b P{GAL4}C673 P{GAL4}C714 P{GAL4}C726b P{GAL4}C789 P{GAL4}C844 P{GawB}bves^C648 P{GawB}c532 P{GawB}c606 P{GawB}jar^C865