Reference Report

Reference
Citation	Scott, M.J., Pan, L.L., Cleland, S.B., Knox, A.L., Heinrich, J. (2000). MSL1 plays a central role in assembly of the MSL complex, essential for dosage compensation in Drosophila. EMBO J. 19(1): 144--155. (Export to RIS)
FlyBase ID	FBrf0123194
Publication Type	Research paper
PubMed ID	10619853
PubMed Abstract	In male Drosophila, histone H4 acetylated at Lys16 is enriched on the X chromosome, and most X-linked genes are transcribed at a higher rate than in females (thus achieving dosage compensation). Five proteins, collectively called the MSLs, are required for dosage compensation and male viability. Here we show that one of these proteins, MSL1, interacts with three others, MSL2, MSL3 and MOF. The latter is a putative histone acetyl transferase. Overexpression of either the N- or C-terminal domain of MSL1 has dominant-negative effects, i.e. causes male-specific lethality. The lethality due to expression of the N-terminal domain is reduced if msl2 is co-overexpressed. MSL2 co-purifies over a FLAG affinity column with the tagged region of MSL1, and both MSL3 and MOF co-purify with the FLAG-tagged MSL1 C-terminal domain. Furthermore, the MSL1 C-terminal domain binds specifically to a GST-MOF fusion protein and co-immunoprecipitates with HA-tagged MSL3. The MSL1 C-terminal domain shows similarity to a region of mouse CBP, a transcription co-activator. We conclude that a main role of MSL1 is to serve as the backbone for assembly of the MSL complex.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Alleles (19)
	mle¹³ mof^hs.PS msl-1^{C.hs.T:Zzzz\FLAG} msl-1^{FC.hs.T:Zzzz\FLAG} msl-1^{FN.hs.T:Zzzz\FLAG} msl-1^hs.PS msl-1^{hs.T:Zzzz\FLAG} msl-1^{MID.hs.T:Zzzz\FLAG} msl-1^Δ84.hs msl-1^{Δ84.T:Zzzz\FLAG} msl-1^Δ84C.hs msl-1^{Δ84C.T:Zzzz\FLAG} msl-1^γ269 msl-2^Hsp83.PK msl-2^γ136 msl-3¹ msl-3^hs.PS msl-3^{hs.T:Ivir\HA1} w^+mC
Constructs (14)
	P{C.msl-1} P{F.msl-1} P{FC.msl-1} P{FN.msl-1} P{FΔ84.msl-1} P{FΔ84C.msl-1} P{H83M2} P{hs-mof} P{hs-msl-1} P{hs-msl-3.HA} P{hs-msl-3} P{MID.msl-1} P{Δ84.msl-1} P{Δ84C.msl-1}
Genes (8)
	mle mof msl-1 msl-2 msl-3 T:Ivir\HA1 T:Zzzz\FLAG w