Reference Report

Reference
Citation	Wan, S., Cato, A.M., Skaer, H. (2000). Multiple signalling pathways establish cell fate and cell number in Drosophila Malpighian tubules. Dev. Biol. 217(1): 153--165. (Export to RIS)
FlyBase ID	FBrf0123233
Publication Type	Research paper
PubMed ID	10625542
PubMed Abstract	A unique cell, the tip mother cell, arises in the primordium of each Drosophila Malpighian tubule by lateral inhibition within a cluster of achaete-expressing cells. This cell maintains achaete expression and divides to produce daughters of equivalent potential, of which only one, the tip cell, adopts the primary fate and continues to express achaete, while in the other, the sibling cell, achaete expression is lost (M. Hoch et al., 1994, Development 120, 3439-3450). In this paper we chart the mechanisms by which achaete expression is differentially maintained in the tip cell lineage to stabilise cell fate. First, wingless is required to maintain the expression of achaete in the tubule primordium so that wingless mutants lack tip cells. Conversely, increasing wingless expression results in the persistence of achaete expression in the cell cluster. Second, Notch signalling is restricted by the asymmetric segregation of Numb, as the tip mother cell divides, so that achaete expression is maintained only in the tip cell. In embryos mutant for Notch tip cells segregate at the expense of sibling cells, whereas in numb neither daughter cell adopts the tip cell fate resulting in tubules with two sibling cells. Conversely, when numb is overexpressed two tip cells segregate and tubules have no sibling cells. Analysis of cell proliferation in the developing tubules of embryos lacking Wingless after the critical period for tip cell allocation reveals an additional requirement for wingless for the promotion of cell division. In contrast, alteration in the expression of numb has no effect on the final tubule cell number.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (2)
	Df(2L)30A-C Df(2R)P72
Alleles (10)
	insc^unspecified N^int.hs nrm^A37 numb¹ numb⁷⁹⁶ numb^Scer\UAS.cWa Scer\GAL4^hs.PB wg^hs.PN wg^l-12 wg^l-17
Constructs (4)
	P{GAL4-Hsp70.PB} P{hsp70-Notch.intra} P{HS-wg} P{UAS-numb.W}
Genes (10)
	ac ct futsch insc Kr N nrm numb Scer\GAL4 wg
Insertions (1)
	P{A92}nrm^A37