Reference Report

Reference
Citation	Zhou, J., Levine, M. (1999). A novel cis-regulatory element, the PTS, mediates an anti-insulator activity in the Drosophila embryo. Cell 99(6): 567--575.
FlyBase ID	FBrf0123260
Type of publication	Research paper
Offprint Available	No
External Crossreferences
PubMed ID	10612393
PubMed Abstract	The Abd-B Hox gene contains an extended 3' cis-regulatory region that is subdivided into a series of separate lab domains. The lab-7 domain activates Abd-B in parasegment 12 (ps12), whereas lab-8 controls expression in ps13. iab-7 is flanked by two insulators, Fab-7 and Fab-8, which are thought to prevent regulatory factors, such as Polycomb silencers, from influencing neighboring iab domains. This organization poses a potential paradox, since insulator DNAs can work in a dominant fashion to block enhancer-promoter interactions over long distances. Here, we present evidence for a novel cis-regulatory sequence located within lab-7, the promoter targeting sequence (PTS), which permits distal enhancers to overcome the blocking effects of Fab-8 and the heterologous su(Hw) insulator. We propose that the PTS converts dominant, long-range insulators into local regulatory elements that separate neighboring lab domains.
Biosis	2000.75989
Zoological record
Associated Information
Comments
Text of personal communication
Associated files
Related Publications
Review	Paper alert. Tsang, 2000, Curr. Opin. Genet. Dev. 10(2): 136 [FBrf0126750]
Also Published As

Other Reference Information
Secondary IDs
Language of publication	English
Additional language(s) of abstract
ISBN
Place of publication
Published In
Abbreviation	Cell
Title	Cell
Authors
Volume range	1-
Year range	1974-
Page range
Publisher	Cell Press
Place of publication	Cambridge, MA
Language of publication	English
ISBN/ISSN	0092-8674
CODEN	CELLB5
Data from Reference
Aberrations (2)
	Df(3R)Abd-B^S10 Dp(3;3)P5
Alleles (33)
	Abd-B-PTS^1.7 Abd-B-PTS⁶²⁵ Abd-B-PTS^tmr Abd-B⁰⁵⁶⁴⁹ Abd-B^iab7-R73 Ecol\lacZ^Abd-B-05649 Ecol\lacZ^{Abd-B.gypsy\su(Hw)BR.AE1} Ecol\lacZ^{Abd-B.gypsy\su(Hw)BR.Scer\FRT.PTS.AE1} Ecol\lacZ^{Abd-B.iab8.1.6} Ecol\lacZ^{H1.Fab-8.IAB5} Ecol\lacZ^H1.Fab-8.NEE Ecol\lacZ^H1.IAB5 Ecol\lacZ^H1.NEE.Z Ecol\lacZ^H1.tmr.NEE Ecol\lacZ^P\T.PC Ecol\lacZ^{gypsy\su(Hw)BR.Scer\FRT.PTS.iab8} Ecol\lacZ^{gypsy\su(Hw)BR.iab8} Ecol\lacZ^{iab8.Scer\FRT.Fab-8.iab7.mKr} Ecol\lacZ^{iab8.iab7.mKr} Fab-8^1.7 Fab-8⁵⁹⁰ Fab-8^tmr gypsy\su(Hw)BR³⁶⁰ iab-8PRE^tmr w^+mC w^{Abd-B.iab7.0.7.mKr} w^{H1.Fab-8.IAB5} w^H1.Fab-8.NEE w^H1.IAB5 w^H1.NEE.Z w^H1.tmr.NEE w^{iab8.Scer\FRT.Fab-8.iab7.mKr} w^{iab8.iab7.mKr}
Constructs (13)
	P{Abd-B-lacZ.gypsy\su(Hw)BR.AE1} P{Abd-B-lacZ.gypsy\su(Hw)BR.Scer\FRT.PTS.AE1} P{H1-Fab-8-IAB5} P{H1-spacer-IAB5} P{lacZ.gypsy\su(Hw)BR.Scer\FRT.PTS.iab8} P{lacZ.gypsy\su(Hw)BR.iab8} P{w-Abd-B.iab7.0.7.mKr-lacZ} P{w-Abd-B.iab8.1.6-lacZ} P{w-H1-lacZ-Fab-8-NEE} P{w-H1-lacZ-NEE} P{w-H1-lacZ.tmr} P{w.lacZ.iab8.Scer\FRT.Fab-8.iab7.mKr} P{w.lacZ.iab8.iab7.mKr}
Genes (7)
	Abd-B Abd-B-PTS Ecol\lacZ Fab-8 gypsy\su(Hw)BR iab-8PRE w
Insertions (1)
	P{PZ}Abd-B⁰⁵⁶⁴⁹