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Citation
Tuschl, T., Zamore, P.D., Lehmann, R., Bartel, D.P., Sharp, P.A. (1999). Targeted mRNA degradation by double-stranded RNA in vitro.  Genes Dev. 13(24): 3191--3197.
FlyBase ID
FBrf0124982
Publication Type
Research paper
Abstract

Double-stranded RNA (dsRNA) directs gene-specific, post-transcriptional silencing in many organisms, including vertebrates, and has provided a new tool for studying gene function. The biochemical mechanisms underlying this dsRNA interference (RNAi) are unknown. Here we report the development of a cell-free system from syncytial blastoderm Drosophila embryos that recapitulates many of the features of RNAi. The interference observed in this reaction is sequence specific, is promoted by dsRNA but not single-stranded RNA, functions by specific mRNA degradation, and requires a minimum length of dsRNA. Furthermore, preincubation of dsRNA potentiates its activity. These results demonstrate that RNAi can be mediated by sequence-specific processes in soluble reactions.

PubMed ID
PubMed Central ID
PMC317199 (PMC) (EuropePMC)
Related Publication(s)
Review

Paper alert.
Aasland, 2000, Curr. Opin. Genet. Dev. 10(2): 132 [FBrf0126740]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genes Dev.
    Title
    Genes & Development
    Publication Year
    1987-
    ISBN/ISSN
    0890-9369
    Data From Reference
    Genes (4)