Personal Communication to FlyBase Received from: Katherine Brendza Washington University School of Medicine Department of Biochemistry and Molecular Biophysics Subject Khc gene annotation Date: March 2, 2000 Query from curator: I am currently working on Khc and wanted to include the sites of the mutations cited in the Brendza et. al. 1999. J.B.C. 274(44):31506-31514 publication in the annotation. I had a bit of trouble matching up the stated locations of the nucleotide and amino acid changes with the actual sequence. My best guess for the actual changes are below. Could you please confirm or correct these. mutation nt change nt change aa change aa change paper my guess paper my guess Khc[8] C955T C948T R219 R210 Khc[4] C1198T C1192T T291M same Khc[2] C1078T G1071A E251K same Khc[10] T1294A A1287T T323S same C1396T G1389A E357K same Khc[37] C1156T G1149A D277N same Khc[36] G1234A C1227T R303C same Khc[32] G720C G720A E134K same Khc[24] C513T same Q65@ same Khc[27] C513T same Q65@ same Khc[23] C817T G810A E164K same Khc[19] G856A G849A E177K same Khc[18] G565A G558A V80I same Khc[17] C1064T C1057T S246F same Reply: I spent the morning going through Joy Yang's sequence and looking at our changes and your guesses are correct based on Joy's published sequence (Yang et.al. 1989. Cell 56:879-889 ). I have listed below the changes that are correct.. mutation nt change aa change Khc[8] C948T R210@ Khc[4] C1192T T291M Khc[2] G1071A E251K Khc[10] A1287T T323S G1389A E357K Khc[37] G1149A D277N Khc[36] C1227T R303C Khc[32] G720A E134K Khc[24] C513T Q65@ Khc[27] C513T Q65@ Khc[23] G810A E164K Khc[19] G849A E177K Khc[18] G558A V80I Khc[17] C1057T S246F
