Home
Reference Report
| Reference | |||
|---|---|---|---|
| Citation | Goyal, L., McCall, K., Agapite, J., Hartwieg, E., Steller, H. (2000). Induction of apoptosis by Drosophila reaper, hid and grim through inhibition of IAP function. EMBO J. 19(4): 589--597. (Export to RIS) | ||
| FlyBase ID | FBrf0125392 | ||
| Publication Type | Research paper | ||
| PubMed ID | 10675328 | ||
| PubMed Abstract | Induction of apoptosis in Drosophila requires the activity of three closely linked genes, reaper, hid and grim. Here we show that the proteins encoded by reaper, hid and grim activate cell death by inhibiting the anti-apoptotic activity of the Drosophila IAP1 (diap1) protein. In a genetic modifier screen, both loss-of-function and gain-of-function alleles in the endogenous diap1 gene were obtained, and the mutant proteins were functionally and biochemically characterized. Gain-of-function mutations in diap1 strongly suppressed reaper-, hid- and grim-induced apoptosis. Sequence analysis of these alleles revealed that they were caused by single amino acid changes in the baculovirus IAP repeat domains of diap1, a domain implicated in binding REAPER, HID and GRIM. Significantly, the corresponding mutant DIAP1 proteins displayed greatly reduced binding of REAPER, HID and GRIM, indicating that REAPER, HID and GRIM kill by forming a complex with DIAP1. These data provide strong in vivo evidence for a previously published model of cell death regulation in Drosophila. | ||
| DOI | |||
| Related Publication(s) | |||
| Review | Paper alert. Schuldt, 2000, Curr. Opin. Genet. Dev. 10(3): 240--241 [FBrf0128842] |
||
Recent Updates
|
|||
| Description |
What does this section display?
This section contains items that were added to this record for each release.
It currently only tracks new links between this FlyBase report and other
FlyBase data classes (e.g. genes, references, stocks) or controlled
vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
|
||
| Update Feed |
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your
feed reader.
|
||
| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
|
Associated Information
|
|||
| Comments | |||
| Associated Files | |||
|
Other Information
|
|||
| Secondary IDs | |||
| Language of Publication | English | ||
| Additional Languages of Abstract | |||
| Also Published As | |||
|
Parent Publication
|
|||
| Publication Type | Journal | ||
| Abbreviation | EMBO J. | ||
| Title | The EMBO Journal | ||
| Publication Year | 1982- | ||
| ISBN/ISSN | 0261-4189 | ||
|
Data from Reference
|
|||
| Aberrations (1)
|
|||
| Alleles (16)
|
|||
| Constructs (2)
|
|||
| Genes (4)
|
|||