Reference Report

Reference
Citation	Rebay, I., Chen, F., Hsiao, F., Kolodziej, P.A., Kuang, B.H., Laverty, T., Suh, C., Voas, M., Williams, A., Rubin, G.M. (2000). A genetic screen for novel components of the Ras/Mitogen-activated protein kinase signaling pathway that interact with the yan gene of Drosophila identifies split ends, a new RNA recognition motif-containing protein. Genetics 154(2): 695--712. (Export to RIS)
FlyBase ID	FBrf0125429
Publication Type	Research paper
External Crossreferences
PubMed ID	10655223
PubMed Abstract	The receptor tyrosine kinase (RTK) signaling pathway is used reiteratively during the development of all multicellular organisms. While the core RTK/Ras/MAPK signaling cassette has been studied extensively, little is known about the nature of the downstream targets of the pathway or how these effectors regulate the specificity of cellular responses. Drosophila yan is one of a few downstream components identified to date, functioning as an antagonist of the RTK/Ras/MAPK pathway. Previously, we have shown that ectopic expression of a constitutively active protein (yan(ACT)) inhibits the differentiation of multiple cell types. In an effort to identify new genes functioning downstream in the Ras/MAPK/yan pathway, we have performed a genetic screen to isolate dominant modifiers of the rough eye phenotype associated with eye-specific expression of yan(ACT). Approximately 190,000 mutagenized flies were screened, and 260 enhancers and 90 suppressors were obtained. Among the previously known genes we recovered are four RTK pathway components, rolled (MAPK), son-of-sevenless, Star, and pointed, and two genes, eyes absent and string, that have not been implicated previously in RTK signaling events. We also isolated mutations in five previously uncharacterized genes, one of which, split ends, we have characterized molecularly and have shown to encode a member of the RRM family of RNA-binding proteins.
BIOSIS ID	2000.136208
Zoological Record ID
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Editors
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (5)
	Df(2L)ast1 Df(2L)net-PM47C Df(2R)CX1 Df(3R)e-R1 In(2LR)spen^XLS1000
Alleles (71)
	aop^ACT.GMR aop^ACT.sev aop^S2382 EY2-5^AH425 EY2-5^DW1080 EY2-6^AC403 EY2-6^AQR618 EY2-8^EP1160 EY2-8^xCB204 EY3-2^xAZ145 EY3-2^xCF301 EY3-2^xDF411 EY3-3^xBE183 EY3-3^xF6 EY3-4^x1S805 EY3-4^xII790 EY3-5^xKR845 EY3-5^xZB970 eya¹ eya^A188 eya^G130 gl¹ gl^DZ1053 gl^ER1085 lilli^DE884 lilli^DR999 lilli^s35 phl¹² pnt^07825Δ78 pnt^AF397 pnt^AP553 Ras85D^N17.sev Ras85D^V12.sev retn¹ retn^B142 retn^x1N793 rl^A0648 rl^AL528 rl^S-135 S^BA651 S^e220 S^U132 Sin3A^CY886 Sin3A^DW1046 Sin3A^k07401 Sos^D120 Sos^e2H Sos^xB10 spen⁰³³⁵⁰ spen^AH393 spen^BU720 spen^k06703a spen^k06805 spen^k07612 spen^k07721 spen^k08102 spen^k10325 spen^k13233 spen^k13601 spen^k13624 spen^k15612 spen^XLS1000 stg^D115 stg^DD937 stg^eAS7a Su(Tpl)^DI998 Su(Tpl)^EZ1217 Su(Tpl)^S-192 Taf4^AG456 Taf4^I53 Taf4^S-117
Balancers (7)
	CyO CyO-GCY CyO-SCY TM3-GTY TM3-Sb TM3-STY TM3-T2B
Constructs (4)
	P{GMR-yan^ACT} P{sevRas1.N17} P{sevRas1.V12} P{sev-yan^ACT}
Genes (24)
	aop EY2-5 EY2-6 EY2-8 EY3-2 EY3-3 EY3-4 EY3-5 eya gl lilli phl pnt Ras85D retn rl S Sin3A Sos spen stg Su(Tpl) SY2-2 Taf4
Insertions (5)
	P{GMR-yan^ACT}IL2 P{GMR-yan^ACT}IL4 P{lacW}spen^k06703a P{sev-yan^ACT}IL1 P{sev-yan^ACT}IL3