Reference Report

Reference
Citation	Andrews, J., Garcia-Estefania, D., Delon, I., Lu, J., Mevel-Ninio, M., Spierer, A., Payre, F., Pauli, D., Oliver, B. (2000). OVO transcription factors function antagonistically in the Drosophila female germline. Development 127(4): 881--892. (Export to RIS)
FlyBase ID	FBrf0125467
Publication Type	Research paper
PubMed ID	10648246
PubMed Abstract	OVO controls germline and epidermis differentiation in flies and mice. In the Drosophila germline, alternative OVO-B and OVO-A isoforms have a common DNA-binding domain, but different N-termini. We show that these isoforms are transcription factors with opposite regulatory activities. Using yeast one-hybrid assays, we identified a strong activation domain within a common region and a counteracting repression domain within the OVO-A-specific region. In flies, OVO-B positively regulated the ovarian tumor promoter, while OVO-A was a negative regulator of the ovarian tumor and ovo promoters. OVO-B isoforms supplied ovo(+) function in the female germline and epidermis, while OVO-A isoforms had dominant-negative activity in both tissues. Moreover, elevated expression of OVO-A resulted in maternal-effect lethality while the absence of OVO-A resulted in maternal-effect sterility. Our data indicate that tight regulation of antagonistic OVO-B and OVO-A isoforms is critical for germline formation and differentiation.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (31)
	Ecol\lacZ^C4.Adh Ecol\lacZ^otu.PA Ecol\lacZ^ovo.1.1 Ecol\lacZ^ovo.Δap Ecol\lacZ^ovo.Δbp en::ovo^{B2.EN.Scer\UAS} en::ovo^{D1.EN.Scer\UAS} en::ovo^D1.EN en::ovo^EN ovo^A.Scer\UAS ovo^amk ovo^amv ovo^B2.Scer\UAS ovo^{B.Scer\UAS.P\T} ovo^D1-7 ovo^{D1.Scer\UAS.cAa} ovo^D1 ovo^D1r+ ovo^D1rv22 ovo^D1rv23 ovo^D2 ovo^D3 ovo^D4 ovo^S-7.2 ovo^svb-1 ovo^Δa2 ovo^Δap Scer\GAL4^e22c Scer\GAL4^{nos.UTR.T:Hsim\VP16} w^+mC w^+mW.hs
Constructs (20)
	P{GAL4::VP16-nos.UTR} P{otu-lacZ.wt} P{ovo^amk} P{ovo^amv} P{ovo^D1.EN} P{ovo^D1-7} P{ovo^D1r+} P{ovo^D4} P{ovo^EN} P{ovo^Δa2} P{ovo^Δap} P{ovo-lacZ.1.1} P{ovo-lacZ.Δap} P{ovo-lacZ.Δbp} P{UAS-en::ovo.B2} P{UAS-en::ovo.D1} P{UAS-ovo.A} P{UAS-ovo.B2} P{UAS-ovo.D1.A} P{UASp-ovo.B}
Genes (6)
	Ecol\lacZ en::ovo ovo Scer\GAL4 T:Hsim\VP16 w
Insertions (1)
	P{en2.4-GAL4}e22c