|Citation||Andrews, J., Garcia-Estefania, D., Delon, I., Lu, J., Mevel-Ninio, M., Spierer, A., Payre, F., Pauli, D., Oliver, B. (2000). OVO transcription factors function antagonistically in the Drosophila female germline. Development 127(4): 881--892. (Export to RIS)|
|Publication Type||Research paper|
|PubMed Abstract||OVO controls germline and epidermis differentiation in flies and mice. In the Drosophila germline, alternative OVO-B and OVO-A isoforms have a common DNA-binding domain, but different N-termini. We show that these isoforms are transcription factors with opposite regulatory activities. Using yeast one-hybrid assays, we identified a strong activation domain within a common region and a counteracting repression domain within the OVO-A-specific region. In flies, OVO-B positively regulated the ovarian tumor promoter, while OVO-A was a negative regulator of the ovarian tumor and ovo promoters. OVO-B isoforms supplied ovo(+) function in the female germline and epidermis, while OVO-A isoforms had dominant-negative activity in both tissues. Moreover, elevated expression of OVO-A resulted in maternal-effect lethality while the absence of OVO-A resulted in maternal-effect sterility. Our data indicate that tight regulation of antagonistic OVO-B and OVO-A isoforms is critical for germline formation and differentiation.|
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|Language of Publication||English|
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