Reference Report

Reference
Citation	Schotta, G., Reuter, G. (2000). Controlled expression of tagged proteins in Drosophila using a new modular P-element vector system. Mol. Gen. Genet. 262(6): 916--920. (Export to RIS)
FlyBase ID	FBrf0125475
Publication Type	Research paper
PubMed ID	10660052
PubMed Abstract	We have developed a new modular vector system that facilitates the combination of various DNA fragments as functional modules for P element-mediated transformation of Drosophila melanogaster. The basic vector pP¿GS¿ contains unique sites for 17 restriction enzymes, including three 8-bp cutters, that allow one to combine various promoter elements, cDNAs and genomic DNA fragments, as well as protein tags and selectable marker genes, for a wide spectrum of transgene analyses. With this new vector system we analysed the chromosomal distribution of the Drosophila SU(VAR)3-9 protein tagged with EGFP, using hsp70-cDNA and genomic Su(var)3-9 constructs. We found preferential association of the tagged SU(VAR)3-9 with centric heterochromatin.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Gen. Genet.
Title	Molecular and General Genetics
Publication Year	1967-2001
ISBN/ISSN	0026-8925
Data from Reference
Alleles (7)
	ry^+t7.2 Scer\UAS^5x.hs.SV40 Su(var)3-9^{10kb.T:Avic\GFP-EGFP} Su(var)3-9^{hs.T:Avic\GFP-EGFP} T:Avic\GFP^BFP T:Avic\GFP^EGFP v^+t1.8
Constructs (29)
	P{GS.ry⁺(10kbSu(var)3-9)EGFP} P{GS.ry⁺} P{GS.ry⁺EGFP} P{GS.ry⁺hs(Su(var)3-9cDNA)EGFP} P{GS.ry⁺hs} P{GS.ry⁺hsEBFP} P{GS.ry⁺hsEGFP} P{GS.ry⁺hslexA} P{GS.ry⁺lexA} P{GS.ry⁺UAS-EGFP} P{GS.v⁺} P{GS.v⁺EGFP} P{GS.v⁺hsEGFP} P{GS.v⁺hslexA} P{GS.v⁺lexA} pP{GS.ry⁺} pP{GS.ry⁺EGFP} pP{GS.ry⁺hs} pP{GS.ry⁺hsEBFP} pP{GS.ry⁺hsEGFP} pP{GS.ry⁺hslexA} pP{GS.ry⁺lexA} pP{GS.ry⁺UAS-EGFP} pP{GS.v⁺} pP{GS.v⁺EGFP} pP{GS.v⁺hsEGFP} pP{GS.v⁺hslexA} pP{GS.v⁺lexA} pP{GS-SR}
Genes (5)
	ry Scer\UAS Su(var)3-9 T:Avic\GFP v