Reference Report

Reference
Citation	Lazzaro, B. (2000.3.9). Helping FlyBase: ADRC-627B. (Export to RIS)
FlyBase ID	FBrf0126873
Publication Type	Personal communication to FlyBase
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PubMed Abstract
Text of Personal Communication	From rd120@XXXXXXXXXXXXXXX Fri Mar 03 10:05:08 2000 To: bplazzaro@XXXXXXXXXXXXXXX Subject: Helping FlyBase: ADRC-627B Dear Brian, We are currently curating the abstracts for the upcoming 41st (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. I am writing in connection with your abstract: 'Variation in Immune Response in Drosophila melanogaster at the Molecular and Phenotypic Levels.' You mention two genes that are new to FlyBase, AttB and AttC. Do you have a map locations for AttB and AttC? These are data items that we track, if possible. Another abstract, 623A (Testing the usefulness of attacin reporter constructs as monitors of 18-wheeler activity in the humoral immune response, by Eldon et al.) describes an attacin B. Do you know whether this is the same gene as your AttB? If they are the same gene they should be represented by one gene record in FlyBase, but if they are not they should be represented by two records - which is why I ask. Thank you for your help, with best wishes, Rachel. From bplazzaro@XXXXXXXXXXXXXXX Thu Mar 09 20:18:50 2000 To: rd120@XXXXXXXXXXXXXXX Subject: Re: Helping FlyBase: ADRC-627B Dear Rachel, I would be happy to provide you with information about Attacin B and Attacin C. I first recovered the sequence that I am referring to as Attacin B by directly sequencing the product of a low-stringency genomic PCR on a line deleted for Attacin A. A BLAST of the recovered sequence against the BDGP database showed that the recovered sequence lies 1136 bases downstream of the known Attacin A gene. BDGP also returned a hit, which I am referring to as Attacin C, in an independent contig. BLASTS against the Celera sequence confirmed the sequences of the two novel genes and suggested that there are no other Attacin genes in the D. melanogaster genome. The BDGP clone containing Attacin C apparently maps around cytological position 50A-51A. Att A and Att B are arranged head to tail: AttA 5'->3' -- <1.1 kb> -- AttB 5'->3'B. I don't know the orientation of AttC relative to AttA/AttB. . Brian Lazzaro _______________________________________________________________________ Brian P. Lazzaro (814) 863-7045 office Department of Biology (814) 865-2715 lab Penn State University (814) 865-9131 fax University Park, PA 16802 bplazzaro@XXXXXXXXXXXXXXX ______________________________________________________________________
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Language of Publication	English
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Genes (3)
	AttA AttB AttC