Reference Report

Reference
Citation	Gao, X., Neufeld, T.P., Pan, D. (2000). Drosophila PTEN regulates cell growth and proliferation through PI3K-dependent and -independent pathways. Dev. Biol. 221(2): 404--418. (Export to RIS)
FlyBase ID	FBrf0127089
Publication Type	Research paper
PubMed ID	10790335
PubMed Abstract	The control of cell and organ growth is fundamental to the development of multicellular organisms. Here, we show that dPTEN, a Drosophila homolog of the mammalian PTEN tumor suppressor gene, plays an essential role in the control of cell size, cell number, and organ size. In mosaic animals, dPTEN(-) cells proliferate faster than their heterozygous siblings, show an autonomous increase in cell size, and form organs of increased size, whereas overexpression of dPTEN results in opposite phenotypes. The loss-of-function phenotypes of dPTEN are suppressed by mutations in the PI3K target Dakt1 and the translational initiation factor eif4A, suggesting that dPTEN acts through the PI3K signaling pathway to regulate translation. Although activation of PI3K and Akt has been reported to increase rates of cellular growth but not proliferation, loss of dPTEN stimulates both of these processes, suggesting that PTEN regulates overall growth through PI3K/Akt-dependent and -independent pathways. Furthermore, we show that dPTEN does not play a major role in cell survival during Drosophila development. Our results provide a potential explanation for the high frequency of PTEN mutation in human cancer.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (15)
	Akt1⁰⁴²²⁶ BacA\p35^Scer\UAS.cHa eIF-4a¹⁰⁰⁶ eIF-4a¹⁰⁶⁹ InR³⁵ InR⁰⁵⁵⁴⁵ Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} Pi3K92E^{Scer\UAS.T:Hsap\MYC} Pten^+t6 Pten^dj189 Pten^Scer\UAS.cGb Scer\GAL4^ap-md544 Scer\GAL4^arm.PS Scer\GAL4^Bx-MS1096 Scer\GAL4^en-e16E
Constructs (6)
	P{Dp110^D954A} P{GAL4-arm.S} P{Pten.6} P{UAS-p35.H} P{UAS-Pten.Gb} P{WT-Dp110}
Genes (9)
	Akt1 BacA\p35 eIF-4a InR Pi3K92E Pten Rsf1 Scer\GAL4 T:Hsap\MYC
Insertions (6)
	F{}Pten^dj189 P{en2.4-GAL4}e16E P{GawB}ap^md544 P{GawB}Bx^MS1096 P{lacW}eIF-4a¹⁰⁰⁶ P{lacW}eIF-4a¹⁰⁶⁹