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Citation
Hoskins, R.A., Nelson, C.R., Berman, B.P., Laverty, T.R., George, R.A., Ciesiolka, L., Naeemuddin, M., Arenson, A.D., Durbin, J., David, R.G., Tabor, P.E., Bailey, M.R., De Shazo, D.R., Catanese, J., Mammoser, A., Osoegawa, K., de Jong, P.J., Celniker, S.E., Gibbs, R.A., Rubin, G.M., Scherer, S.E. (2000). A BAC-based physical map of the major autosomes of Drosophila melanogaster.  Science 287(5461): 2271--2274.
FlyBase ID
FBrf0127123
Publication Type
Research paper
Abstract
We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Science
    Title
    Science
    Publication Year
    1895-
    ISBN/ISSN
    0036-8075
    Data From Reference
    Datasets (5)
    Molecular Constructs (1)