Reference Report

Reference
Citation	Lee, T., Winter, C., Marticke, S.S., Lee, A., Luo, L. (2000). Essential roles of Drosophila RhoA in the regulation of neuroblast proliferation and dendritic but not axonal morphogenesis. Neuron 25(2): 307--316. (Export to RIS)
FlyBase ID	FBrf0127188
Publication Type	Research paper
PubMed ID	10719887
PubMed Abstract	The pleiotropic functions of small GTPase Rho present a challenge to its genetic analysis in multicellular organisms. We report here the use of the MARCM (mosaic analysis with a repressible cell marker) system to analyze the function of RhoA in the developing Drosophila brain. Clones of cells homozygous for null RhoA mutations were specifically labeled in the mushroom body (MB) neurons of mosaic brains. We found that RhoA is required for neuroblast (Nb) proliferation but not for neuronal survival. Surprisingly, RhoA is not required for MB neurons to establish normal axon projections. However, neurons lacking RhoA overextend their dendrites, and expression of activated RhoA causes a reduction of dendritic complexity. Thus, RhoA is an important regulator of dendritic morphogenesis, while distinct mechanisms are used for axonal morphogenesis.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Neuron
Title	Neuron
Publication Year	1988-
ISBN/ISSN	0896-6273
Data from Reference
Alleles (10)
	Khc::nod^{Scer\UAS.T:Ecol\lacZ} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} n-syb^{Scer\UAS.cEa.T:Avic\GFP} Rho1^72O Rho1^72R Rho1^Scer\UAS.cMa Rho1^V14.Scer\UAS Scer\GAL4^elav-C155 Scer\GAL4^Tab2-201Y Scer\GAL80^αTub84B.PL
Constructs (7)
	P{tubP-GAL80} P{UAS-Khc::nod::lacZ} P{UAS-mCD8::GFP.L} P{UAS-n-syb.GFP} P{UAS-n-syb-GFP.E} P{UAS-Rho1.Sph} P{UAS-Rho1.V14}
Genes (11)
	CycB Khc::nod Lam Mmus\Cd8a n-syb Rho1 Scer\GAL4 Scer\GAL80 T:Avic\GFP T:Ecol\lacZ α-Spec
Insertions (2)
	P{GawB}elav^C155 P{GawB}Tab2^201Y