Reference Report

Reference
Citation	Li, H., Chaney, S., Forte, M., Hirsh, J. (2000). Ectopic G-protein expression in dopamine and serotonin neurons blocks cocaine sensitization in Drosophila melanogaster. Curr. Biol. 10(4): 211--214. (Export to RIS)
FlyBase ID	FBrf0127196
Publication Type	Research paper
PubMed ID	10704417
PubMed Abstract	Sensitization to repeated doses of psychostimulants is thought to be an important component underlying the addictive process in humans [1] [2] [3] [4]. In all vertebrate animal models, including humans [5], and even in fruit flies, sensitization is observed after repeated exposure to volatilized crack cocaine [6]. In vertebrates, sensitization is thought to be initiated by processes occurring in brain regions that contain dopamine cell bodies [2] [7]. Here, we show that modulated cell signaling in the Drosophila dopamine and serotonin neurons plays an essential role in cocaine sensitization. Targeted expression of either a stimulatory (Galpha(s)) or inhibitory (Galpha(i)) Galpha subunit, or tetanus toxin light chain (TNT) in dopamine and serotonin neurons of living flies blocked behavioral sensitization to repeated cocaine exposures. These flies showed alterations in their initial cocaine responsiveness that correlated with compensatory adaptations of postsynaptic receptor sensitivity. Finally, repeated drug stimulation of a nerve cord preparation that is postsynaptic to the brain amine cells failed to induce sensitization, further showing the importance of presynaptic modulation in sensitization.
DOI
Related Publication(s)
Erratum	Erratum. Ectopic G-protein expression in dopamine and serotonin neurons blocks cocaine sensitization in Drosophila melanogaster. Li et al., 2000, Curr. Biol. 10(10): R393 [FBrf0128835]
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Secondary IDs	FBrf0126734
Language of Publication	English
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Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Alleles (4)
	Ctet\TeTxLC^TNT.Scer\UAS Gαi^Scer\UAS.cLa Gαs^Scer\UAS.cCa Scer\GAL4^Ddc.PL
Constructs (4)
	P{Ddc-GAL4.L} P{UAS-G-iα65A.L} P{UAS-G-sα60A.C} P{UAS-TeTxLC.tnt}
Genes (5)
	Ctet\TeTxLC Ddc Gαi Gαs Scer\GAL4
Transcripts (1)
	Scer\GAL4^Ddc.PLRA