Reference Report

Reference
Citation	Mollereau, B., Wernet, M.F., Beaufils, P., Killian, D., Pichaud, F., Kuhnlein, R., Desplan, C. (2000). A green fluorescent protein enhancer trap screen in Drosophila photoreceptor cells. Mech. Dev. 93(1-2): 151--160. (Export to RIS)
FlyBase ID	FBrf0127238
Publication Type	Research paper
PubMed ID	10781948
PubMed Abstract	The Drosophila ommatidia contain two classes of photoreceptor cells (PR's), the outer and the inner PR's. We performed an enhancer trap screen in order to target genes specifically expressed in PR's. Using the UAS/GAL4 method with enhanced green fluorescent protein (eGFP) as a vital marker, we screened 180000 flies. Out of 2730 lines exhibiting new eGFP patterns, we focused on 16 lines expressing eGFP in particular subsets of PR's. In particular, we describe three lines inserted near the spalt major, m-spondin and furrowed genes, whose respective expression patterns resemble those genes. These genes had not been reported to be expressed in the adult eye. These examples clearly show the ability of our screen to target genes expressed in the adult Drosophila eye.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Mech. Dev.
Title	Mechanisms of Development
Publication Year	1990-
ISBN/ISSN	0925-4773
Data from Reference
Alleles (28)
	Avic\GFP^{E.Scer\UAS.cMa} Ecol\lacZ^Scer\UAS.cBa fw^POc2.1 mspo^R7TS12.7 salm^R7T3.8 Scer\GAL4^B8.6.4 Scer\GAL4^fw-POc2.1 Scer\GAL4^{mspo-R7TS12.7} Scer\GAL4^ninaE.PT Scer\GAL4^OmBrA6.9 Scer\GAL4^P9.2 Scer\GAL4^PAMLBr5.21 Scer\GAL4^PB7.30 Scer\GAL4^PR2.27 Scer\GAL4^PRAb12.7 Scer\GAL4^R1-6AM12.16 Scer\GAL4^R1-6H12.6 Scer\GAL4^R1-6M12.17 Scer\GAL4^R1-6OcB1.20 Scer\GAL4^R1-6T12.22 Scer\GAL4^R8.2.3 Scer\GAL4^Rh4.PT Scer\GAL4^Rh5.PT Scer\GAL4^salm-R7T3.8 Scer\GAL4^waw-PTA Scer\GAL4^wB w^+mC waw^PTA
Constructs (5)
	P{GawB} P{rh1-GAL4} P{Rh4-GAL4} P{Rh5-GAL4} P{UAS-EGFP.M}
Genes (8)
	Avic\GFP Ecol\lacZ fw mspo salm Scer\GAL4 w waw
Insertions (17)
	P{GawB}B8.6.4 P{GawB}fw^POc2.1 P{GawB}mspo^R7TS12.7 P{GawB}OmBrA6.9 P{GawB}P9.2 P{GawB}PAMLBr5.21 P{GawB}PB7.30 P{GawB}PR2.27 P{GawB}PRAb12.7 P{GawB}R1-6AM12.16 P{GawB}R1-6H12.6 P{GawB}R1-6M12.17 P{GawB}R1-6OcB1.20 P{GawB}R1-6T12.22 P{GawB}R8.2.3 P{GawB}salm^R7T3.8 P{GawB}waw^PTA
Transcripts (1)
	Scer\GAL4^PR2.27RA