Reference Report

Reference
Citation	Sanchez Soriano, N., Russell, S. (2000). Regulatory mutations of the Drosophila Sox gene Dichaete reveal new functions in embryonic brain and hindgut development. Dev. Biol. 220(2): 307--321. (Export to RIS)
FlyBase ID	FBrf0127303
Publication Type	Research paper
External Crossreferences
PubMed ID	10753518
PubMed Abstract	Sox domain proteins encompass a conserved family of transcriptional regulators that are implicated in a variety of developmental processes in eukaryotes from worm to man. The Dichaete gene of Drosophila encodes a group B Sox protein related to mammalian Sox1, -2, and -3 and, like these proteins, it is widely and dynamically expressed throughout embryogenesis. In order to unravel new Dichaete functions, we characterized the organization of the Dichaete gene using a combination of regulatory mutant alleles and reporter gene constructs. Dichaete expression is tightly controlled during embryonic development by a complex of regulatory elements distributed over 25 kb downstream and 3 kb upstream of the transcription unit. A series of regulatory alleles which affect tissue-specific domains of Dichaete were used to demonstrate that Dichaete has functions in addition to those during segmentation and midline development previously described. First, Dichaete has functions in the developing brain. A specific group of neural cells in the tritocerebrum fails to develop correctly in the absence of Dichaete, as revealed by reduced expression of labial, zfh-2, wingless, and engrailed. Second, Dichaete is required for the correct differentiation of the hindgut. The Dichaete requirement in hindgut morphogenesis is, in part, via regulation of dpp, since ectopically supplied dpp can rescue Dichaete phenotypes in the hindgut. Taken together, there are now four distinct in vivo functions described for Dichaete that can be used as models for context-dependent comparative studies of Sox function.Copyright 2000 Academic Press.
BIOSIS ID	2000.255462
Zoological Record ID
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Editors
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (9)
	Df(3L)D⁵ Df(3L)fz-GS1a In(3L)D In(3L)D³ In(3L)D¹⁰ In(3L)D^r8 In(3LR)D⁶ T(2;3)D⁴ T(2;3)D⁷
Alleles (20)
	D¹ D³ D⁴ D⁵ D⁶ D⁷ D¹⁰ D^r8 D^r321 D^Scer\UAS.cSa D^unspecified dpp^Scer\UAS.cSa Ecol\lacZ^cas-1530 Ecol\lacZ^D3'1 Ecol\lacZ^D3'2 Ecol\lacZ^D3'3 Ecol\lacZ^D5'1 hh^Scer\UAS.cCa Scer\GAL4^en-e16E w^+mC
Constructs (7)
	P{D3'1} P{D3'2} P{D3'3} P{D5'1} P{UAS-D.S} P{UAS-dpp.S} P{UAS-hh.C}
Genes (13)
	cas D dpp Ecol\lacZ en hh l(1)sc lab Scer\GAL4 w wg zfh1 α-Spec
Insertions (3)
	P{en2.4-GAL4}e16E P{lacW}cas¹⁵³⁰ P{PZ}rJ375
Polypeptides (1)
	D-XP
Transcripts (4)
	Ecol\lacZ^D3'1RA Ecol\lacZ^D3'2RA Ecol\lacZ^D3'3RA Ecol\lacZ^D5'1RA