Reference Report

Reference
Citation	Takatsu, Y., Nakamura, M., Stapleton, M., Danos, M.C., Matsumoto, K., O'Connor, M.B., Shibuya, H., Ueno, N. (2000). TAK1 participates in c-Jun N-terminal kinase signaling during Drosophila development. Mol. Cell. Biol. 20(9): 3015--3026. (Export to RIS)
FlyBase ID	FBrf0127351
Publication Type	Research paper
PubMed ID	10757786
PubMed Abstract	Transforming growth factor beta (TGF-beta)-activated kinase 1 (TAK1) is a member of the MAPKKK superfamily and has been characterized as a component of the TGF-beta/bone morphogenetic protein signaling pathway. TAK1 function has been extensively studied in cultured cells, but its in vivo function is not fully understood. In this study, we isolated a Drosophila homolog of TAK1 (dTAK1) which contains an extensively conserved NH(2)-terminal kinase domain and a partially conserved COOH-terminal domain. To learn about possible endogenous roles of TAK1 during animal development, we generated transgenic flies which express dTAK1 or the mouse TAK1 (mTAK1) gene in the fly visual system. Ectopic activation of TAK1 signaling leads to a small eye phenotype, and genetic analysis reveals that this phenotype is a result of ectopically induced apoptosis. Genetic and biochemical analyses also indicate that the c-Jun amino-terminal kinase (JNK) signaling pathway is specifically activated by TAK1 signaling. Expression of a dominant negative form of dTAK during embryonic development resulted in various embryonic cuticle defects including dorsal open phenotypes. Our results strongly suggest that in Drosophila melanogaster, TAK1 functions as a MAPKKK in the JNK signaling pathway and participates in such diverse roles as control of cell shape and regulation of apoptosis.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Aberrations (2)
	Df(3L)H99 In(2L)dpp^d6
Alleles (57)
	aos²⁵⁷ BacA\p35^GMR.PH bi^omb-3198 bsk¹ bsk² bsk^flp147E Dl^RF dpp^d6 dpp^d12 Dsor1^LH110 Dsor1^r2 Dsor1^Su1 Ecol\lacZ^Scer\UAS.cUa Egfr^E3 Egfr^f6 hep¹ hep^r75 hep^Scer\UAS.cGa Hsap\TAB1^GMR.PT Iap2^GMR.PH Mad¹² Med⁴ Mmus\Tak1^GMR.PT Mmus\Tak1^K63W.GMR Mmus\Tak1^Scer\UAS.cTa Mmus\Tak1^ΔN.GMR Mmus\Tak1^ΔN.Scer\UAS N^55e11 phl¹ put¹³⁵ put¹⁰⁴⁶⁰ Ras85D^e2F RasGAP1^B2 rl^Sem sax¹ Scer\GAL4^Dll-md23 Scer\GAL4^elav-C155 Scer\GAL4^en-e16E Scer\GAL4^GMR.PF Scer\GAL4^pnr-MD237 Scer\GAL4^unspecified scw^E2 sev¹⁴ shn¹ shn⁰⁴⁷³⁸ Sos^34Ea-7 Sos^JC2 spi¹ Tak1^{K46R.Scer\UAS} Tak1^Scer\UAS.cTa th^GMR.PH tkv⁵ tkv⁷ tkv⁸ tld¹⁵ tld^B4 tld^E4
Constructs (14)
	P{GAL4-ninaE.GMR} P{GMR-DIAP1} P{GMR-DIAP2} P{GMR-hTAB1} P{GMR-mTAK1.K63W} P{GMR-mTAK1.ΔN} P{GMR-mTAK1} P{GMRP35} P{UAS-hep.G} P{UAS-lacZ.U} P{UAS-mTAK1.ΔN} P{UAS-mTAK1} P{UAS-Tak1.K46R} P{UAS-Tak1}
Genes (35)
	aos BacA\p35 bi bsk Dl dpp Dsor1 Ecol\lacZ Egfr hep Hsap\TAB1 Iap2 Mad Med Mmus\Tak1 N phl puc put Ras85D RasGAP1 rl rpr sax Scer\GAL4 scw sev shn Sos spi Tak1 th tkv tld W
Insertions (6)
	?{}N^55e11 P{en2.4-GAL4}e16E P{GawB}Dll^md23 P{GawB}elav^C155 P{GawB}pnr^MD237 P{PZ}shn⁰⁴⁷³⁸
Transcripts (1)
	Ecol\lacZ^puc-E69RA