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Abdelilah-Seyfried, S., Chan, Y.M., Zeng, C., Justice, N.J., Younger-Shepherd, S., Sharp, L.E., Barbel, S., Meadows, S.A., Jan, L.Y., Jan, Y.N. (2000). A gain-of-function screen for genes that affect the development of the Drosophila adult external sensory organ.  Genetics 155(2): 733--752.
FlyBase ID
FBrf0128367
Publication Type
Research paper
Abstract

The Drosophila adult external sensory organ, comprising a neuron and its support cells, is derived from a single precursor cell via several asymmetric cell divisions. To identify molecules involved in sensory organ development, we conducted a tissue-specific gain-of-function screen. We screened 2293 independent P-element lines established by P. Rorth and identified 105 lines, carrying insertions at 78 distinct loci, that produced misexpression phenotypes with changes in number, fate, or morphology of cells of the adult external sensory organ. On the basis of the gain-of-function phenotypes of both internal and external support cells, we subdivided the candidate lines into three classes. The first class (52 lines, 40 loci) exhibits partial or complete loss of adult external sensory organs. The second class (38 lines, 28 loci) is associated with increased numbers of entire adult external sensory organs or subsets of sensory organ cells. The third class (15 lines, 10 loci) results in potential cell fate transformations. Genetic and molecular characterization of these candidate lines reveals that some loci identified in this screen correspond to genes known to function in the formation of the peripheral nervous system, such as big brain, extra macrochaetae, and numb. Also emerging from the screen are a large group of previously uncharacterized genes and several known genes that have not yet been implicated in the development of the peripheral nervous system.

PubMed ID
PubMed Central ID
PMC1461115 (PMC) (EuropePMC)
DOI
Related Publication(s)
Erratum

A gain-of-function screen for genes that affect the development of the Drosophila adult external sensory organ.
Abdelilah-Seyfried et al., 2001, Genetics 157(1): 457 [FBrf0132338]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Alleles (113)
    Genes (92)
    Insertions (116)
    Transgenic Constructs (1)