Reference Report

Reference
Citation	Bui, Q.T., Zimmerman, J.E., Liu, H., Bonini, N.M. (2000). Molecular analysis of Drosophila eyes absent mutants reveals features of the conserved eya domain. Genetics 155(2): 709--720. (Export to RIS)
FlyBase ID	FBrf0128419
Publication Type	Research paper
PubMed ID	10835393
PubMed Abstract	The eyes absent (eya) gene is critical to eye formation in Drosophila; upon loss of eya function, eye progenitor cells die by programmed cell death. Moreover, ectopic eya expression directs eye formation, and eya functionally synergizes in vivo and physically interacts in vitro with two other genes of eye development, sine oculis and dachshund. The Eya protein sequence, while highly conserved to vertebrates, is novel. To define amino acids critical to the function of the Eya protein, we have sequenced eya alleles. These mutations have revealed that loss of the entire Eya Domain is null for eya activity, but that alleles with truncations within the Eya Domain display partial function. We then extended the molecular genetic analysis to interactions within the Eya Domain. This analysis has revealed regions of special importance to interaction with Sine Oculis or Dachshund. Select eya missense mutations within the Eya Domain diminished the interactions with Sine Oculis or Dachshund. Taken together, these data suggest that the conserved Eya Domain is critical for eya activity and may have functional subregions within it.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (31)
	dac^Scer\UAS.cSa eya¹ eya² eya^cli-IID eya^E1 eya^E2 eya^E3 eya^E4 eya^E5 eya^E6 eya^E7.Scer\UAS eya^E7 eya^E8 eya^E9 eya^E10 eya^E11.Scer\UAS eya^E11 eya^E12 eya^E(P)10 eya^IR1 eya^IR3 eya^Scer\UAS.cBa eya^Z283 Mmus\Eya1^Scer\UAS.cBa Mmus\Eya2^Scer\UAS.cBa Mmus\Eya3^Scer\UAS.cBa Scer\GAL4^dpp.blk1 Scer\GAL4^ey.PB Scer\GAL4^GMR.PF so^Scer\UAS.cPa w^+mC
Constructs (11)
	P{GAL4-dpp.blk1} P{GAL4-ey.B} P{GAL4-ninaE.GMR} P{UAS-dac.S} P{UAS-eya.B.I} P{UAS-eya.E7} P{UAS-eya.E11} P{UAS-Mmus\Eya1.B} P{UAS-Mmus\Eya2.B} P{UAS-Mmus\Eya3.B} P{UAS-so.P}
Genes (8)
	dac eya Mmus\Eya1 Mmus\Eya2 Mmus\Eya3 Scer\GAL4 so w