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Reference
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| Citation |
Locke, J., Podemski, L., Aippersbach, N., Kemp, H., Hodgetts, R. (2000). A physical map of the polytenized region (101EF-102F) of chromosome 4 in Drosophila melanogaster. Genetics 155(3): 1175--1183. |
| FlyBase ID |
FBrf0128554 |
| Type of publication |
Research paper |
| Offprint Available |
Yes |
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External Crossreferences
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| PubMed ID |
10880479 |
| PubMed Abstract |
Chromosome 4, the smallest autosome ( approximately 5 Mb in length) in Drosophila melanogaster contains two major regions.
The centromeric domain ( approximately 4 Mb) is heterochromatic and consists primarily of short, satellite repeats. The remaining
approximately 1.2 Mb, which constitutes the banded region (101E-102F) on salivary gland polytene chromosomes and contains
the identified genes, is the region mapped in this study. Chromosome walking was hindered by the abundance of moderately repeated
sequences dispersed along the chromosome, so we used many entry points to recover overlapping cosmid and BAC clones. In situ
hybridization of probes from the two ends of the map to polytene chromosomes confirmed that the cloned region had spanned
the 101E-102F interval. Our BAC clones comprised three contigs; one gap was positioned distally in 102EF and the other was
located proximally at 102B. Twenty-three genes, representing about half of our revised estimate of the total number of genes
on chromosome 4, were positioned on the BAC contigs. A minimal tiling set of the clones we have mapped will facilitate both
the assembly of the DNA sequence of the chromosome and a functional analysis of its genes.
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| Biosis |
2000.349277 |
| Zoological record |
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Associated Information
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| Comments |
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| Text of personal communication |
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Related Publications
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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Published In
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| Abbreviation |
Genetics |
| Title |
Genetics |
| Authors |
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| Volume range |
1- |
| Year range |
1916- |
| Page range |
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| Publisher |
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| Place of publication |
Austin, TX, etc |
| Language of publication |
English |
| ISBN/ISSN |
0016-6731 |
| CODEN |
GENTAE |
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Data from Reference
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| Aberrations (1)
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| Genes (27)
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| Insertions (1)
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| Natural transposons (2)
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