Reference Report

Reference
Citation	Martin-Bermudo, M.D. (2000). Integrins modulate the Egfr signaling pathway to regulate tendon cell differentiation in the Drosophila embryo. Development 127(12): 2607--2615. (Export to RIS)
FlyBase ID	FBrf0128565
Publication Type	Research paper
PubMed ID	10821759
PubMed Abstract	Changes in the extracellular matrix (ECM) govern the differentiation of many cell types during embryogenesis. Integrins are cell matrix receptors that play a major role in cell-ECM adhesion and in transmitting signals from the ECM inside the cell to regulate gene expression. In this paper, it is shown that the PS integrins are required at the muscle attachment sites of the Drosophila embryo to regulate tendon cell differentiation. The analysis of the requirements of the individual alpha subunits, alphaPS1 and alphaPS2, demonstrates that both PS1 and PS2 integrins are involved in this process. In the absence of PS integrin function, the expression of tendon cell-specific genes such as stripe and beta1 tubulin is not maintained. In addition, embryos lacking the PS integrins also exhibit reduced levels of activated MAPK. This reduction is probably due to a downregulation of the Epidermal Growth Factor receptor (Egfr) pathway, since an activated form of the Egfr can rescue the phenotype of embryos mutant for the PS integrins. Furthermore, the levels of the Egfr ligand Vein at the muscle attachment sites are reduced in PS mutant embryos. Altogether, these results lead to a model in which integrin-mediated adhesion plays a role in regulating tendon cell differentiation by modulating the activity of the Egfr pathway at the level of its ligand Vein.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (16)
	btl::Egfr^{Scer\UAS.T:λ\cI-DD} Ecol\lacZ^sr-B14.0 Egfr^DN.Scer\UAS if^B4 mew^M6 mew^{Scer\UAS.cMBa} mew^{Δcyt.Scer\UAS} mys::tor^D.Scer\UAS mys¹¹ mys^din.Scer\UAS Scer\GAL4^69B Scer\GAL4^how-24B Scer\GAL4^twi.PG spi^s.Scer\UAS T:λ\cI^DD vn^Scer\UAS.cYa
Constructs (9)
	P{GAL4-twi.G} P{UAS-Egfr.DN} P{UAS-Egfr.λtop} P{UAS-mew.MB} P{UAS-mew.Δcyt} P{UAS-mys::tor.D} P{UAS-mys.din} P{UAS-spi.s} P{UAS-vn.Y}
Genes (14)
	btl::Egfr Ecol\lacZ Egfr if mew mys mys::tor rl Scer\GAL4 spi T:λ\cI Tig vn βTub56D
Insertions (3)
	P{GawB}69B P{GawB}how^24B P{lwB}sr^B14.0