Reference Report

Reference
Citation	Wang, Q.T., Holmgren, R.A. (2000). Nuclear import of Cubitus interruptus is regulated by Hedgehog via a mechanism distinct from Ci stabilization and Ci activation. Development 127(14): 3131--3139. (Export to RIS)
FlyBase ID	FBrf0128675
Publication Type	Research paper
PubMed ID	10862750
PubMed Abstract	The Hedgehog (Hh) signal is transduced via Cubitus interruptus (Ci) to specify cell fates in the Drosophila wing. In the absence of Hh, the 155 kDa full-length form of Ci is cleaved into a 75 kDa repressor. Hh inhibits the proteolysis of full-length Ci and facilitates its conversion into an activator. Recently, it has been suggested that Hh promotes Ci nuclear import in tissue culture cells. We have studied the mechanism of Ci nuclear import in vivo and the relationship between nuclear import, stabilization and activation. We found that Ci rapidly translocates to the nucleus in cells close to the anteroposterior (AP) boundary and this rapid nuclear import requires Hh signaling. The nuclear import of Ci is regulated by Hh even under conditions in which Ci is fully stabilized. Furthermore, cells that exhibit Ci stabilization and rapid nuclear import do not necessarily exhibit maximal Ci activity. It has been previously shown that stabilization does not suffice for activation. Consistent with this finding, our results suggest that the mechanisms regulating nuclear import, stabilization and activation are distinct from each other. Finally, we show that cos2 and pka, two molecules that have been characterized primarily as negative regulators of Ci activity, also have positive roles in the activation of Ci in response to Hh.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (15)
	ci^{m1-3.Scer\UAS.T:Ivir\HA1} ci^Scer\UAS.cHb cos¹ Ecol\lacZ^ci.sd.4bs fu¹ fu⁹⁴ fu^mH63 fu^RX15 Pka-C1^H2 ptc⁹ ptc^G20 Scer\GAL4^71B Scer\GAL4^ptc-559.1 smo¹ smo^D16
Constructs (3)
	P{4bs-lacZ} P{UAS-ci-cDNA} P{UAS-HA.ci.m1-3}
Genes (9)
	ci cos Ecol\lacZ fu Pka-C1 ptc Scer\GAL4 smo T:Ivir\HA1
Insertions (2)
	P{GawB}71B P{GawB}ptc^559.1