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Citation	Sigrist, S.J., Thiel, P.R., Reiff, D.F., Lachance, P.E.D., Lasko, P., Schuster, C.M. (2000). Postsynaptic translation affects the efficacy and morphology of neuromuscular junctions. Nature 405(6790): 1062--1065.
FlyBase ID	FBrf0128816
Type of publication	Research paper
Offprint Available	Yes
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PubMed ID	10890448
PubMed Abstract	Long-term synaptic plasticity may be associated with structural rearrangements within the neuronal circuitry. Although the molecular mechanisms governing such activity-controlled morphological alterations are mostly elusive, polysomal accumulations at the base of developing dendritic spines and the activity-induced synthesis of synaptic components suggest that localized translation is involved during synaptic plasticity. Here we show that large aggregates of translational components as well as messenger RNA of the postsynaptic glutamate receptor subunit DGluR-IIA are localized within subsynaptic compartments of larval neuromuscular junctions of Drosophila melanogaster. Genetic models of junctional plasticity and genetic manipulations using the translation initiation factors eIF4E and poly(A)-binding protein showed an increased occurrence of subsynaptic translation aggregates. This was associated with a significant increase in the postsynaptic DGluR-IIA protein levels and a reduction in the junctional expression of the cell-adhesion molecule Fasciclin II. In addition, the efficacy of junctional neurotransmission and the size of larval neuromuscular junctions were significantly increased. Our results therefore provide evidence for a postsynaptic translational control of long-term junctional plasticity.
Biosis	2000.404099
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Language of publication	English
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Abbreviation	Nature
Title	Nature
Authors
Volume range	1-
Year range	1869-
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Place of publication	London
Language of publication	English
ISBN/ISSN	0028-0836
CODEN	NATUAS
Data from Reference
Aberrations (2)
	Df(2L)cl-h4 Df(2R)Pcl7B
Alleles (15)
	GluRIIA^g9 M(2)24F¹ M(2)36F^unspecified M(2)53¹ M(2)58F^unspecified Scer\GAL4^Mhc.PW Scer\GAL4^elav.PLu Sh^unspecified dnc^unspecified eIF-4E^Scer\UAS.cRa eag^unspecified pAbp^EP310 pAbp^Scer\UAS.cSa pAbp^k10109 w^+mC
Constructs (4)
	P{GAL4-Mhc.W} P{GAL4-elav.L} P{UAS-eIF-4E.R} P{UAS-pAbp.S}
Genes (15)
	Fas2 GluRIIA M(2)24F M(2)36F M(2)53 M(2)58F Mhc Scer\GAL4 Sh dnc eIF-4E eag pAbp syt w
Insertions (2)
	P{EP}pAbp^EP310 P{lacW}pAbp^k10109