Reference Report

Reference
Citation	Roote, J. (2000.6.13). New P derivatives. (Export to RIS)
FlyBase ID	FBrf0129261
Publication Type	Personal communication to FlyBase
PubMed ID
PubMed Abstract
Text of Personal Communication	From jr32@XXXXXXXXXXXXXXX Tue Jun 13 19:18:51 2000 To: rd120@XXXXXXXXXXXXXXX Subject: new P derivatives Dear Rachel, Here is a list of deletions that we have made over the last few years by P-element excision and male recombination - some very informative, some less so. All induced with Δ2-3. Fondest regards, Rootles (pers comm that if you dare) k07245 BG:DS00797.1 k07245-rv1 kuz^-, l(2)34Db⁺. rN149 BG:DS08220.1 rn149-rv3 RpII33⁺, Ance^-, j^-, rk^-, l(2)34Fa^-, wb^-, ms(2)34Fe⁺ k11509 smi35A k11509-rv18 wb^- k08712 k08712-rv2 l(2)34Fd^-, semi-lethal, short bristles k08712-rv21 l(2)35Aa^-, lethal, rescued by 35Aa 5kb rescue fragment k08712-rv34 l(2)34Fd^-, lethal EP(2)2039 elB EP(2)2039-rv4 wb⁺, ms(2)34Fe^-,l(2)34Fc^-, l(2)34Fd^-, l(2)35Aa^-, elB^-, pu⁺ EP(2)2039-rv3 elB^-, pu^- k13218 osp 225 w^- revertants 7 were osp^-, 3 were noc^+/- osp^- k11524 l(2)35Bb 2 out of 7 excisions revert the lethality. k08808 l(2)35Bc 121 excisions, 37 lethal over Df(2L)nBR107 (i.e. 84 precise excisions): 31 imprecise excisions l(2)35Bc^-, 5 deletions: k08808-rv25 l(2)35Be⁺, l(2)35Bc^-, l(2)35Bd^-, l(2)35Bg⁺ k08808-rv52 l(2)35Be⁺, l(2)35Bc^-, l(2)35Bd^-, l(2)35Bg⁺ k08808-rv57 l(2)35Be⁺, l(2)35Bc^-, l(2)35Bd^-, l(2)35Bg⁺ k08808-rv63 l(2)35Be⁺, l(2)35Bc^-, l(2)35Bd^-, l(2)35Bg⁺ k08808-rv70 l(2)35Bf⁺, l(2)35Be^-, l(2)35Bc^-, l(2)35Bd⁺ Therefore the gene order is 35Bb, 35Bf, 35Be, 35Bc, 35Bd. PZ05271 l(2)35Ea 1 excision out of 14 (from Julie Gates, Utah) is lethal. PZ05271-rv54 l(2)35Ea^- k09834 PRL-1 k09834^rv1 Ca-a1⁺, twe^-, crp^-, l(2)35Fb⁺ k09834^rv2 Ca-a1⁺, twe^-, crp⁺ k17027 cact k17027^rv1 fzy⁺, cact^- (fs over H60-3), l(2)35Fe⁺ k17027^rv2 cact⁺, l(2)35Fe^-, chif⁺ k17027^rv3 fzy⁺, cact^- (fs over cact^AR51.4), l(2)35Fe⁺ k04216 chif RN2 chif^WD18 k04216-rv1 female sterile female fertile! k04216-rv2 female sterile female sterile k04216-rv3 female sterile female sterile k08106 multiple insert in 35F 14 excisions, selected as w^-: 10 lethal, 4 semi-lethal and fs over RN2, 6 retain weak w⁺. k08106-rv4 cni⁺, fzy^-, cact^-, l(2)35Fe^-, chif^-, l(2)35Fg^-, dac⁺; w⁺ (weak) k08106-rv6 cni⁺, fzy^-, cact^-, l(2)35Fe^-, chif^-, l(2)35Fg^-, dac⁺; w^- k08106-rv15 cni⁺, fzy^-, cact^-, l(2)35Fe^-, chif^-, l(2)35Fg^-, dac⁺; w^- Male recombinants - induced over dp^ov1 b¹ cn¹ bw¹ k07245 BG:DS00797.1 k07245-mr2 b kuz⁺, l(2)34Db^- - Ance^-. k07245-mr9 dp kuz^+/- k07245-mr11 b kuz⁺, l(2)34Db^- - wb^- k07245-mr28 dp kuz^+/-, l(2)34Db⁺ k07245-mr31 b kuz⁺, l(2)34Db^- - ms(2)35Ci^-, esg⁺ k07245-mr32 b kuz⁺, l(2)34Db^- - gft^-, rd⁺ k05007 CycE k05007-rv1 cn bw CycE⁺, l(2)35Di^-, l(2)35Df⁺ mr1 cn bw lace⁺, CycE^-, l(2)35Di^-, l(2)35Df⁺ mr2 cn bw lace⁺, CycE^-, l(2)35Di^-, l(2)35Df^-, Gli^-, l(2)35Ea⁺ mr3 cn bw lace⁺, CycE^-, l(2)35Di^-, l(2)35Df^-, Gli^-, l(2)35Ea⁺ mr6 cn bw lace⁺, CycE^-, l(2)35Di^-, l(2)35Df^-, Gli^-, l(2)35Ea⁺ mr9 cn bw lace⁺, CycE^-, l(2)35Di^-, l(2)35Df^-, Gli^-, l(2)35Ea⁺ mr13 cn bw lace⁺, CycE^-, l(2)35Di^-, l(2)35Df^-, Gli^-, l(2)35Ea⁺ mr15 cn bw lace⁺, CycE^-, l(2)35Di^-, l(2)35Df^-, Gli^-, l(2)35Ea^-, l(2)35Dh^-, l(2)35De^- mr19 dp b vasa⁺, stc^- - CycE^-, l(2)35Di⁺ PZ07130 ck PZ07130-mr1 dp b kuz^- - ck^-, l(2)35Cf⁺ PZ07130-mr2 cn bw Su(H)⁺, ck^- - vasa^-, stc⁺ PZ07130-mr3 cn bw Su(H)⁺, ck^-, l(2)35Cf⁺ PZ07130-mr5 cn bw Su(H)⁺, ck^- - esg^-, wor⁺ PZ07130-mr7 cn bw Su(H)⁺, ck^- - vasa^-, stc⁺ PZ07130-mr8 cn bw Su(H)⁺, ck^-, l(2)35Cf⁺ PZ07130-mr9 dp b l(2)35Bg^- - ck^-, l(2)35Cf⁺ PZ06430 gft PZ06430-mr1 cn bw l(2)35Cc⁺, gft^-, ms(2)35Ci⁺ mr2 dp b l(2)35Cc⁺, gft^-, ms(2)35Ci⁺ mr3 cn bw l(2)35Cc⁺, gft^-, ms(2)35Ci^-, esg⁺ mr4 cn bw l(2)35Cc⁺, gft^-, ms(2)35Ci^-, esg⁺ mr5 cn bw l(2)35Cc⁺, gft^-, ms(2)35Ci^-, esg⁺ mr7 cn bw l(2)35Cc⁺, gft^-, ms(2)35Ci^-, esg⁺ mr8 cn bw l(2)35Cc⁺, gft^-, ms(2)35Ci⁺ mr9 cn bw l(2)35Cc⁺, gft^-, ms(2)35Ci^-, esg⁺ mr10 cn bw l(2)35Cc⁺, gft^-, ms(2)35Ci^-, esg⁺ mr11 dp b l(2)35Cc⁺, gft^-, ms(2)35Ci⁺ mr12 cn bw l(2)35Cc⁺, gft^-, ms(2)35Ci^-, esg⁺ mr13 cn bw l(2)35Cc⁺, gft^-, ms(2)35Ci⁺ mr14 dp b rd⁺, l(2)35Cc, gft^-, ms(2)35Ci⁺ PZ05271 l(2)35Ea PZ05271-mr15 dp b Gli⁺, l(2)35Ea^-, l(2)35De^-, l(2)35Dh^-, BicC⁺ mr33 cn bw CycE⁺, l(2)35Di^-, l(2)35Df^-, Gli^-, l(2)35Ea⁺ mr45 dp b l(2)35Ea^- Note: The dp b deletion should go distally, cn bw proximally. However 35Di, 35Df and Gli are definitely distal of 35Ea. k09033 Gli k09033-mr9 dp b CycE⁺, l(2)35Di^- - Gli^-, l(2)35Ea⁺ k09033-mr19 dp b vasa⁺, stc^- - Gli^-, l(2)35Ea⁺ k14608 l(2)35Fe k14608-mr4 dp b l(2)35Fe^- mr6 dp b l(2)35Di^- - l(2)35Fe^- mr15 dp b fzy^- - l(2)35Fe^- mr16 dp b twe^- - l(2)35Fe^- mr23 cn bw l(2)35Fe^- - chif^- mr27 dp b cact^- - l(2)35Fe^- mr29 dp b l(2)35Ff^- - l(2)35Fe Double-P male recombinants - induced over dp^ov1 b¹ cn¹ bw¹ k07245 - k05605 BG:DS00797.1 - RpII33 k07245L k05605R-mr1 kuz - RpII33 k07245L k05605R-mr3 kuz - RpII33 k07245L k05605R-mr5 l(2)34Db - MtPolB k07245L k05605R-mr9 l(2)34Db - MtPolB k07245L k05605R-mr6 l(2)34Db - l(2)34Fa k07245L k05605R-mr8 l(2)34Db - l(2)34Fa k07245L k05605R-mr10 l(2)34Db - RpII33 k07245L k05605R-mr12 l(2)34Db - RpII33 k07245L k05605R-mr7 l(2)34Db - RpII33 k07245 - k00811 BG:DS00797.1 - l(2)34Fa k07245L k00811R-mr2 l(2)34Db - l(2)34Fa k07245L k00811R-mr3 l(2)34Db - l(2)34Fa k07245L k00811R-mr4 l(2)34Db - l(2)34Fa k07245L k00811R-mr5 l(2)34Db - l(2)34Fa k07245L k00811R-mr11 l(2)34Db - l(2)34Fa k07245L k00811R-mr13 l(2)34Db - l(2)34Fa k07245L k00811R-mr14 l(2)34Db - l(2)34Fa k07245L k00811R-mr6 l(2)34Db - l(2)34Fa k07245L k00811R-mr15 l(2)34Db - l(2)34Fa k07245L k00811R-mr7 l(2)34Db - l(2)34Fa k07245L k00811R-mr8 l(2)34Db - l(2)34Fa k07245L k00811R-mr10 l(2)34Db - l(2)34Fa k07245L k00811R-mr16 l(2)34Db - l(2)34Fa k07245L k00811R-mr17 l(2)34Db - l(2)34Fa w11P - PZ07130 rk - ck rkw11P^L PZ07130^R-mr1 rk - ck rkw11PL PZ07130R-mr2 rk - ck rkw11PL PZ07130R-mr3 rk - ck rkw11PL PZ07130R-mr4 rk - ck rkw11PL PZ07130R-mr5 rk - ck rkw11PL PZ07130R-mr6 rk - ck rkw11PL PZ07130R-mr7 rk - ck rkw11PL PZ07130R-mr8 rk - ck rkw11PL PZ07130R-mr9 rk - ck rkw11PL PZ07130R-mr10 rk - ck rkw11PL PZ07130R-mr11 rk - ck rkw11PL PZ07130R-mr12 rk - ck rkw11PL PZ07130R-mr13 rk - ck rkw11PL PZ07130R-mr14 rk - ck rkw11PL PZ07130R-mr15 rk - ck rkw11PL PZ07130R-mr16 rk - ck
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Aberrations (111)
	Df(2L)EP(2)2039-rv3 Df(2L)EP(2)2039-rv4 Df(2L)k04216-rv1 Df(2L)k04216-rv2 Df(2L)k04216-rv3 Df(2L)k05007-mr1 Df(2L)k05007-mr2 Df(2L)k05007-mr3 Df(2L)k05007-mr6 Df(2L)k05007-mr9 Df(2L)k05007-mr13 Df(2L)k05007-mr15 Df(2L)k05007-mr19 Df(2L)k05007-rv1 Df(2L)k07245^Lk00811^R-mr2 Df(2L)k07245^Lk00811^R-mr3 Df(2L)k07245^Lk00811^R-mr4 Df(2L)k07245^Lk00811^R-mr5 Df(2L)k07245^Lk00811^R-mr6 Df(2L)k07245^Lk00811^R-mr7 Df(2L)k07245^Lk00811^R-mr8 Df(2L)k07245^Lk00811^R-mr10 Df(2L)k07245^Lk00811^R-mr11 Df(2L)k07245^Lk00811^R-mr13 Df(2L)k07245^Lk00811^R-mr14 Df(2L)k07245^Lk00811^R-mr15 Df(2L)k07245^Lk00811^R-mr16 Df(2L)k07245^Lk00811^R-mr17 Df(2L)k07245^Lk05605^R-mr1 Df(2L)k07245^Lk05605^R-mr3 Df(2L)k07245^Lk05605^R-mr5 Df(2L)k07245^Lk05605^R-mr6 Df(2L)k07245^Lk05605^R-mr7 Df(2L)k07245^Lk05605^R-mr8 Df(2L)k07245^Lk05605^R-mr9 Df(2L)k07245^Lk05605^R-mr10 Df(2L)k07245^Lk05605^R-mr12 Df(2L)k07245-mr2 Df(2L)k07245-mr9 Df(2L)k07245-mr11 Df(2L)k07245-mr28 Df(2L)k07245-mr31 Df(2L)k07245-mr32 Df(2L)k07245-rv1 Df(2L)k08106-rv4 Df(2L)k08106-rv6 Df(2L)k08106-rv15 Df(2L)k08712-rv2 Df(2L)k08712-rv21 Df(2L)k08712-rv34 Df(2L)k08808-rv25 Df(2L)k08808-rv52 Df(2L)k08808-rv57 Df(2L)k08808-rv63 Df(2L)k08808-rv70 Df(2L)k09033-mr9 Df(2L)k09033-mr19 Df(2L)k09834^rv1 Df(2L)k09834^rv2 Df(2L)k11509-rv18 Df(2L)k14608-mr4 Df(2L)k14608-mr6 Df(2L)k14608-mr15 Df(2L)k14608-mr16 Df(2L)k14608-mr23 Df(2L)k14608-mr27 Df(2L)k14608-mr29 Df(2L)k17027^rv1 Df(2L)k17027^rv2 Df(2L)k17027^rv3 Df(2L)PZ05271-mr15 Df(2L)PZ05271-mr33 Df(2L)PZ05271-mr45 Df(2L)PZ05271-rv54 Df(2L)PZ06430-mr1 Df(2L)PZ06430-mr2 Df(2L)PZ06430-mr3 Df(2L)PZ06430-mr4 Df(2L)PZ06430-mr5 Df(2L)PZ06430-mr7 Df(2L)PZ06430-mr8 Df(2L)PZ06430-mr9 Df(2L)PZ06430-mr10 Df(2L)PZ06430-mr11 Df(2L)PZ06430-mr12 Df(2L)PZ06430-mr13 Df(2L)PZ06430-mr14 Df(2L)PZ07130-mr1 Df(2L)PZ07130-mr2 Df(2L)PZ07130-mr3 Df(2L)PZ07130-mr5 Df(2L)PZ07130-mr7 Df(2L)PZ07130-mr8 Df(2L)PZ07130-mr9 Df(2L)rkw11P^LPZ07130^R-mr1 Df(2L)rkw11P^LPZ07130^R-mr2 Df(2L)rkw11P^LPZ07130^R-mr3 Df(2L)rkw11P^LPZ07130^R-mr4 Df(2L)rkw11P^LPZ07130^R-mr5 Df(2L)rkw11P^LPZ07130^R-mr6 Df(2L)rkw11P^LPZ07130^R-mr7 Df(2L)rkw11P^LPZ07130^R-mr8 Df(2L)rkw11P^LPZ07130^R-mr9 Df(2L)rkw11P^LPZ07130^R-mr10 Df(2L)rkw11P^LPZ07130^R-mr11 Df(2L)rkw11P^LPZ07130^R-mr12 Df(2L)rkw11P^LPZ07130^R-mr13 Df(2L)rkw11P^LPZ07130^R-mr14 Df(2L)rkw11P^LPZ07130^R-mr15 Df(2L)rkw11P^LPZ07130^R-mr16 Df(2L)rN149-rv3
Alleles (13)
	b¹ bw¹ cact^AR51.4 chif¹ cn¹ dp^ov1 kuz^mr9 kuz^mr28 l(2)35Bc^k08808 mol^k11524a osp^k13218 Pgant35A^+tGal wek^05271a
Constructs (1)
	P{CaGal}
Genes (57)
	Ance b BicC bw cact Ca-α1D chif ck cn cni crp Cul-3 CycE Cyp303a1 dac DNApol-γ35 dp dyn-p25 elB esg fzy Gli kuz l(2)34Db l(2)34Fc l(2)34Fd l(2)35Bc l(2)35Bd l(2)35Be l(2)35Bg l(2)35Cc l(2)35De l(2)35Df l(2)35Di l(2)35Fg lace mol mRpL4 ms(2)34Fe ms(2)35Ci NimA NimC3 osp pburs Pgant35A rd rk RpII33 stc Su(H) Syx5 TfIIS twe vas wb wek wor
Insertions (25)
	P{EP}elB^EP2039 P{lacW}anon-35F-36Aa^k08106g P{lacW}cact^k08106f P{lacW}cact^k17027 P{lacW}CG4455^k04216 P{lacW}chif^k08106h P{lacW}crp^k08106b P{lacW}CycE^k05007 P{lacW}Gli^k09033 P{lacW}l(2)35Bc^k08808 P{lacW}l(2)35Fg^k08106a P{lacW}mol^k11524a P{lacW}mRpL4^k14608 P{lacW}NimC3^k00811 P{lacW}osp^k13218 P{lacW}PRL-1^k09834 P{lacW}Rab14^k08712 P{lacW}RpII33^k05605 P{lacW}smi35A^k11509 P{lacW}TM9SF4^k07245 P{PZ}CenG1A^rN149 P{PZ}ck⁰⁷¹³⁰ P{PZ}Cul-3⁰⁶⁴³⁰ P{PZ}wek^05271a P{wA^R}rk^11P