Reference Report

Reference
Citation	Datar, S.A., Jacobs, H.W., de La Cruz, A.F., Lehner, C.F., Edgar, B.A. (2000). The Drosophila cyclin D-cdk4 complex promotes cellular growth. EMBO J. 19(17): 4543--4554. (Export to RIS)
FlyBase ID	FBrf0129782
Publication Type	Research paper
PubMed ID	10970848
PubMed Abstract	Mammalian cyclin D-Cdk4 complexes have been characterized as growth factor-responsive cell cycle regulators. Their levels rise upon growth factor stimulation, and they can phosphorylate and thus neutralize Retinoblastoma (Rb) family proteins to promote an E2F-dependent transcriptional program and S-phase entry. Here we characterize the in vivo function of Drosophila Cyclin D (CycD). We find that Drosophila CycD-Cdk4 does not act as a direct G(1)/S-phase regulator, but instead promotes cellular growth (accumulation of mass). The cellular response to CycD-Cdk4-driven growth varied according to cell type. In undifferentiated proliferating wing imaginal cells, CycD-Cdk4 caused accelerated cell division (hyperplasia) without affecting cell cycle phasing or cell size. In endoreplicating salivary gland cells, CycD-Cdk4 caused excessive DNA replication and cell enlargement (hypertrophy). In differentiating eyes, CycD-Cdk4 caused cell enlargement (hypertrophy) in post-mitotic cells. Interaction tests with a Drosophila Rb homolog, RBF, indicate that CycD-Cdk4 can counteract the cell cycle suppressive effects of RBF, but that its growth promoting activity is mediated at least in part via other targets.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Alleles (18)
	Avic\GFP^{S65T.Scer\UAS.T:SV40\nls2} Avic\GFP^{S65T.Ubi-p63E.T:SV40\nls2} cdc2c^{Scer\UAS.T:Hsap\MYC} Cdk4^{D175N.Scer\UAS.T:Hsap\MYC} Cdk4^Scer\UAS.cMa CycD^Scer\UAS.cDa CycD^Scer\UAS.cMa CycE^Scer\UAS.cLa Dp^Scer\UAS.cDa E2f^Scer\UAS.cNa Rbf¹⁴ Rbf^Scer\UAS.cDa Scer\GAL4^Act5C.PP Scer\GAL4^ey.PH Scer\GAL4^F4 Scer\GAL4^GMR.PF Scer\GAL4^hs.2sev Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}
Constructs (16)
	P{GAL4-Act5C(FRT.CD2).P} P{GAL4-Act5C(-FRT).P} P{GAL4-ey.H} P{GAL4-Hsp70.sev} P{GAL4-ninaE.GMR} P{UAS-Cdk2-myc} P{UAS-Cdk4.M} P{UAS-Cdk4^D175N-myc} P{UAS-CycD.D} P{UAS-CycD.M} P{UAS-CycE.L} P{UAS-Dp.D} P{UAS-E2f.N} P{UAS-GFP^S65T.nls} P{UAS-Rbf.D} P{Ubi-GFP(S65T)nls}
Genes (11)
	Avic\GFP cdc2c Cdk4 CycD CycE Dp E2f Rbf Scer\GAL4 T:Hsap\MYC T:SV40\nls2
Insertions (11)
	P{GAL4-Act5C(FRT.CD2).P}III P{GAL4-ninaE.GMR}II P{GawB}F4 P{UAS-Cdk4.M}II.1 P{UAS-CycE.L}III P{UAS-Dp.D}III P{UAS-E2f.N}III P{UAS-GFP^S65T.nls}II P{UAS-GFP^S65T.nls}III P{UAS-Rbf.D}III P{Ubi-GFP(S65T)nls}27