| Citation |
Firth, L., Manchester, J., Lorenzen, J.A., Baron, M., Perkins, L.A. (2000). Identification of genomic regions that interact with a viable allele of the Drosophila protein tyrosine phosphatase corkscrew. Genetics 156(2): 733--748. |
| PubMed Abstract |
Signaling by receptor tyrosine kinases (RTKs) is critical for a multitude of developmental decisions and processes. Among
the molecules known to transduce the RTK-generated signal is the nonreceptor protein tyrosine phosphatase Corkscrew (Csw).
Previously, Csw has been demonstrated to function throughout the Drosophila life cycle and, among the RTKs tested, Csw is
essential in the Torso, Sevenless, EGF, and Breathless/FGF RTK pathways. While the biochemical function of Csw remains to
be unambiguously elucidated, current evidence suggests that Csw plays more than one role during transduction of the RTK signal
and, further, the molecular mechanism of Csw function differs depending upon the RTK in question. The isolation and characterization
of a new, spontaneously arising, viable allele of csw, csw(lf), has allowed us to undertake a genetic approach to identify
loci required for Csw function. The rough eye and wing vein gap phenotypes exhibited by adult flies homo- or hemizygous for
csw(lf) has provided a sensitized background from which we have screened a collection of second and third chromosome deficiencies
to identify 33 intervals that enhance and 21 intervals that suppress these phenotypes. We have identified intervals encoding
known positive mediators of RTK signaling, e.g., drk, dos, Egfr, E(Egfr)B56, pnt, Ras1, rolled/MAPK, sina, spen, Src64B, Star,
Su(Raf)3C, and vein, as well as known negative mediators of RTK signaling, e.g., aos, ed, net, Src42A, sty, and su(ve). Of
particular interest are the 5 lethal enhancing intervals and 14 suppressing intervals for which no candidate genes have been
identified.
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