Reference Report

Reference
Citation	Gloor, G.B., Moretti, J., Mouyal, J., Keeler, K.J. (2000). Distinct P-element excision products in somatic and germline cells of Drosophila melanogaster. Genetics 155(4): 1821--1830. (Export to RIS)
FlyBase ID	FBrf0129827
Publication Type	Research paper
PubMed ID	10924477
PubMed Abstract	The footprints remaining following somatic P-element excision from the Drosophila white locus were recovered and characterized. Two different types of footprints were observed. Over 75% of the footprints were short, composed of 4 or 7 nucleotides of the P-element inverted terminal repeat, and were similar to those found in a previously described plasmid excision assay. The remaining footprints were composed of 14-18 nucleotides of both inverted terminal repeats. These large footprints were indistinguishable from those recovered following germline P-element excision. Enhanced expression of the Drosophila homologue of the Ku70 protein did not affect the structure of the somatic footprints. Therefore, this protein is not a limiting factor for double-strand break repair by nonhomologous end-joining in Drosophila somatic cells.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (21)
	Irbp^+t13 Irbp^hs.PR w^11E4 w^+* w^hd80k17 w^hdrvA w^hdrvB w^hdrvC w^hdrvD w^hdrvE w^hdrvF w^hdrvG w^hdrvH w^hdrvI w^hdrvJ w^hdrvK w^hdrvL w^hdrvM w^hdrvN w^hdrvO w^hdrvP
Constructs (2)
	P{gIRBP13} P{hs-Irbp.R}
Genes (3)
	Blm Irbp w
Insertions (2)
	P{gIRBP13}23.1 P{hs-Irbp.R}b-11
Natural transposons (1)
	P-element