Reference Report

Reference
Citation	Tsuji, T., Sato, A., Hiratani, I., Taira, M., Saigo, K., Kojima, T. (2000). Requirements of Lim1, a Drosophila LIM-homeobox gene, for normal leg and antennal development. Development 127(20): 4315--4323. (Export to RIS)
FlyBase ID	FBrf0130121
Publication Type	Research paper
PubMed ID	11003832
PubMed Abstract	During Drosophila leg development, the distal-most compartment (pretarsus) and its immediate neighbour (tarsal segment 5) are specified by a pretarsus-specific homeobox gene, aristaless, and tarsal-segment-specific Bar homeobox genes, respectively; the pretarsus/tarsal-segment boundary is formed by antagonistic interactions between Bar and pretarsus-specific genes that include aristaless (Kojima, T., Sato, M. and Saigo, K. (2000) Development 127, 769-778). Here, we show that Drosophila Lim1, a homologue of vertebrate Lim1 encoding a LIM-homeodomain protein, is involved in pretarsus specification and boundary formation through its activation of aristaless. Ectopic expression of Lim1 caused aristaless misexpression, while aristaless expression was significantly reduced in Lim1-null mutant clones. Pretarsus Lim1 expression was negatively regulated by Bar and abolished in leg discs lacking aristaless activity, which was associated with strong Bar misexpression in the presumptive pretarsus. No Lim1 misexpression occurred upon aristaless misexpression. The concerted function of Lim1 and aristaless was required to maintain Fasciclin 2 expression in border cells and form a smooth pretarsus/tarsal-segment boundary. Lim1 was also required for femur, coxa and antennal development.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(2L)al
Alleles (13)
	al¹ al^ex al^ice al^Scer\UAS.cKa B-H1^Scer\UAS.cSa Ecol\lacZ^arm.PV Ecol\lacZ^Lim1-P0092 Lim1^7B2 Lim1^P0092 Lim1^Scer\UAS.cTa Scer\GAL4^dpp.blk1 Scer\GAL4^ptc-559.1 w^+mC
Constructs (5)
	P{arm-lacZ.V} P{GAL4-dpp.blk1} P{UAS-al.K} P{UAS-B-H1.S} P{UAS-Lim1.T}
Genes (8)
	al B-H1 Dll Ecol\lacZ Fas2 Lim1 Scer\GAL4 w
Insertions (6)
	P{GAL4-dpp.blk1}40C.6 P{GawB}ptc^559.1 P{lArB}Lim1^P0092 P{UAS-al.K}6 P{UAS-B-H1.S}M6 P{UAS-Lim1.T}f111F