Reference Report

Reference
Citation	Yun, B., Lee, K., Farkas, R., Hitte, C., Rabinow, L. (2000). The LAMMER protein kinase encoded by the Doa locus of Drosophila is required in both somatic and germline cells and is expressed as both nuclear and cytoplasmic isoforms throughout development. Genetics 156(2): 749--761. (Export to RIS)
FlyBase ID	FBrf0130184
Publication Type	Research paper
PubMed ID	11014821
PubMed Abstract	Activity of the Darkener of apricot (Doa) locus of Drosophila melanogaster is required for development of the embryonic nervous system, segmentation, photoreceptor maintenance, normal transcription, and sexual differentiation. The gene encodes a protein kinase, with homologues throughout eukaryotes known as the LAMMER kinases. We show here that DOA is expressed as at least two different protein isoforms of 105 and 55 kD throughout development, which are primarily localized to the cytoplasm and nucleus, respectively. Doa transcripts and protein are expressed in all cell types both during embryogenesis and in imaginal discs. Although it was recently shown that DOA kinase is essential for normal sexual differentiation, levels of both kinase isoforms are equal between the sexes during early pupal development. The presence of the kinase on the cell membrane and in the nuclei of polytene salivary gland cells, as well as exclusion from the nuclei of specific cells, may be indicative of regulated kinase localization. Mosaic analysis in both the soma and germline demonstrates that Doa function is essential for cell viability. Finally, in contrast to results reported in other systems and despite some phenotypic similarities, genetic data demonstrate that the LAMMER kinases do not participate in the ras-MAP kinase signal transduction pathway.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (5)
	Df(3L)R Df(3R)3450 In(3)Msu¹ In(3)Msu² In(3R)Doa^3B
Alleles (21)
	Doa^3B Doa⁴ Doa⁷ Doa⁹ Doa^01705b Doa^Dem Doa^HD Doa^RemγA E(gl)3C^unspecified Ecol\lacZ^Doa-01705b Egfr^E1 gl² gl^60j R^unspecified Ras85D^e2F rl¹ rl^Sem sev¹¹ sev¹⁴ sina¹ svp¹
Genes (13)
	Doa E(gl)3C Ecol\lacZ Egfr elav gl R Ras85D rl sev sina svp w
Insertions (1)
	P{PZ}Doa^01705b