Reference Report

Reference
Citation	de Nooij, J.C., Graber, K.H., Hariharan, I.K. (2000). Expression of the cyclin-dependent kinase inhibitor Dacapo is regulated by Cyclin E. Mech. Dev. 97(1-2): 73--83. (Export to RIS)
FlyBase ID	FBrf0130200
Publication Type	Research paper
PubMed ID	11025208
PubMed Abstract	The Cip/Kip family of cyclin-dependent kinase inhibitors (CKIs) has been implicated in mediating cell cycle arrest prior to terminal differentiation. In many instances, increased expression of CKIs immediately precedes mitotic arrest. However, the mechanism that activates CKI expression in cells that are about to stop dividing has remained elusive. Here we have addressed this issue by investigating the expression pattern of dacapo, a Cip/Kip CKI in Drosophila. We show that the accumulation of dacapo RNA and protein requires Cyclin E and that increased expression of Cyclin E can induce dacapo expression. We also show that the oscillation of the Cyclin E and Dacapo proteins are tightly coupled during ovarian endocycles. Our results argue for a mechanism where Cyclin E/Cdk activity induces Dacapo expression but only within certain windows that are permissive for dacapo expression.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mech. Dev.
Title	Mechanisms of Development
Publication Year	1990-
ISBN/ISSN	0925-4773
Data from Reference
Alleles (15)
	CycA⁵ CycE⁰¹⁶⁷² CycE^AR95 CycE^I.hs.2sev CycE^Scer\UAS.cLa Dp^a2 Dp^a4 Dp^GMR.PD E2f⁹¹ E2f¹⁵⁵ E2f^GMR.PD E2f^Scer\UAS.cNa Ecol\lacZ^dap.PdN Scer\GAL4^en-e16E stg²
Constructs (6)
	P{dap-lacZ.dN} P{GMRdDP} P{GMRdE2F} P{sev.CycE.I} P{UAS-CycE.L} P{UAS-E2f.N}
Genes (9)
	CycA CycE dap Dp E2f Ecol\lacZ Rca1 Scer\GAL4 stg
Insertions (3)
	P{en2.4-GAL4}e16E P{hsneo}CycA⁵ P{PZ}CycE⁰¹⁶⁷²