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Cook, K. (2000.10.9). P{PZ}TlrK344
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FBrf0131219
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Personal communication to FlyBase
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From kcook@XXXX Mon Oct 09  20:29:21  2000
To: flybase-updates@XXXX
Subject: P{PZ}TlrK344
From: Kevin Cook, Bloomington Stock Center
Subject: P{PZ}TlrK344
The proximity of the P{PZ} insertion in the 'l(3)rK344' line (FBti0009958)
to the Toll gene (BDGP data) suggested that it was a mutant Tl
allele. Complementation tests against Df(3R)Tl-X, Df(3R)Tl-P, Df(3R)Tl-I
and Df(3R)ME61 showed no lethality; however, females laid eggs that did not
hatch. Complementation tests against Tlr3 and Tlr4 also produced
sterile females. Examination of eggs from the Tlr3 complementation test
showed dorsalized embryos. Consequently, the genotype of the 'l(3)rK344'
chromosome should be changed from P{PZ}l(2)rK344rK344 to P{PZ}TlrK344,
l(3)rK344rK344 to indicate that the P insertion is associated with Tl and
that the lethality is not associated with the P insertion.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-855-5782
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-3700 kcook@XXXX
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    English
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    Aberrations (5)
    Alleles (4)
    Genes (2)
    Insertions (1)