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Citation
Green, C., Levashina, E., McKimmie, C., Dafforn, T., Reichhart, J.M., Gubb, D. (2000). The necrotic gene in Drosophila corresponds to one of a cluster of three serpin transcripts mapping at 43A1.2.  Genetics 156(3): 1117--1127.
FlyBase ID
FBrf0131303
Publication Type
Research paper
Abstract

Mutants of the necrotic (nec) gene in Drosophila melanogaster die in the late pupal stage as pharate adults, or hatch as weak, but relatively normal-looking, flies. Adults develop black melanized spots on the body and leg joints, the abdomen swells with hemolymph, and flies die within 3 or 4 days of eclosion. The TOLL-mediated immune response to fungal infections is constitutively activated in nec mutants and pleiotropic phenotypes include melanization and cellular necrosis. These changes are consistent with activation of one or more proteolytic cascades. The nec gene corresponds to Spn43Ac, one of a cluster of three putative serine proteinase inhibitors at 43A1.2, on the right arm of chromosome 2. Although serpins have been implicated in the activation of many diverse pathways, lack of an individual serpin rarely causes a detectable phenotype. Absence of Spn43Ac, however, gives a clear phenotype, which will allow a mutational analysis of critical features of the molecular structure of serpins.

PubMed ID
PubMed Central ID
PMC1461326 (PMC) (EuropePMC)
DOI
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Aberrations (7)
    Alleles (11)
    Gene Groups (1)
    Genes (6)
    Experimental Tools (1)
    Transgenic Constructs (6)