Reference Report

Reference
Citation	Green, C., Levashina, E., McKimmie, C., Dafforn, T., Reichhart, J.M., Gubb, D. (2000). The necrotic gene in Drosophila corresponds to one of a cluster of three serpin transcripts mapping at 43A1.2. Genetics 156(3): 1117--1127. (Export to RIS)
FlyBase ID	FBrf0131303
Publication Type	Research paper
PubMed ID	11063688
PubMed Abstract	Mutants of the necrotic (nec) gene in Drosophila melanogaster die in the late pupal stage as pharate adults, or hatch as weak, but relatively normal-looking, flies. Adults develop black melanized spots on the body and leg joints, the abdomen swells with hemolymph, and flies die within 3 or 4 days of eclosion. The TOLL-mediated immune response to fungal infections is constitutively activated in nec mutants and pleiotropic phenotypes include melanization and cellular necrosis. These changes are consistent with activation of one or more proteolytic cascades. The nec gene corresponds to Spn43Ac, one of a cluster of three putative serine proteinase inhibitors at 43A1.2, on the right arm of chromosome 2. Although serpins have been implicated in the activation of many diverse pathways, lack of an individual serpin rarely causes a detectable phenotype. Absence of Spn43Ac, however, gives a clear phenotype, which will allow a mutational analysis of critical features of the molecular structure of serpins.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (7)
	Df(2R)nap2 Df(2R)pk78k Df(2R)pk³⁰ Df(2R)pk-sple-51 Df(2R)sple-D1 Df(2R)sple-D2 In(1)w^m4
Alleles (11)
	nec¹ nec² nec^+t7.0 nec^PP.Scer\UAS nec^Scer\UAS.cLa pk³⁰ Scer\GAL4^da.G32 Spn43Aa^t3.2 Spn43Ab³⁰ Spn43Ab^t5.2 spz⁴
Constructs (6)
	P{GAL4-da.G32} P{nec.7.0.G} P{Spn43Aa.3.2.G} P{Spn43Aa.5.2.G} P{UAS-nec.L} P{UAS-nec^PP.G}
Genes (6)
	nec pk Scer\GAL4 Spn43Aa Spn43Ab spz