Reference Report

Reference
Citation	Huang, A.M., Rubin, G.M. (2000). A misexpression screen identifies genes that can modulate RAS1 pathway signaling in Drosophila melanogaster. Genetics 156(3): 1219--1230. (Export to RIS)
FlyBase ID	FBrf0131317
Publication Type	Research paper
External Crossreferences
PubMed ID	11063696
PubMed Abstract	Differentiation of the R7 photoreceptor cell is dependent on the Sevenless receptor tyrosine kinase, which activates the RAS1/mitogen-activated protein kinase signaling cascade. Kinase suppressor of Ras (KSR) functions genetically downstream of RAS1 in this signal transduction cascade. Expression of dominant-negative KSR (KDN) in the developing eye blocks RAS pathway signaling, prevents R7 cell differentiation, and causes a rough eye phenotype. To identify genes that modulate RAS signaling, we screened for genes that alter RAS1/KSR signaling efficiency when misexpressed. In this screen, we recovered three known genes, Lk6, misshapen, and Akap200. We also identified seven previously undescribed genes; one encodes a novel rel domain member of the NFAT family, and six encode novel proteins. These genes may represent new components of the RAS pathway or components of other signaling pathways that can modulate signaling by RAS. We discuss the utility of gain-of-function screens in identifying new components of signaling pathways in Drosophila.
BIOSIS ID	2001.21028
Zoological Record ID
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Editors
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (25)
	Akap200^EP2254 Akap200^Scer\UAS.cHa chb^EP3403 ksr::tor^{ΔNksr.hs.sev} Lk6^EP886 Lk6^EP3094 Lk6^Scer\UAS.cHa MESK1^EP980 MESK2^EP2347 MESK3^EP3728 MESK4^EP1015 MESR3^EP2221 MESR4^EP386 MESR6^EP3142 MESR6^Scer\UAS.cHa msn^EP609 msn^Scer\UAS.cSa NFAT^EP1353 NFAT^Scer\UAS.cHa Ras85D^V12.sev Scer\GAL4^Act5C.PI Scer\GAL4^dpp.blk1 Scer\GAL4^hs.2sev Scer\GAL4^vg.PB w^+mC
Constructs (11)
	P{Act5C-GAL4} P{GAL4-dpp.blk1} P{GAL4-Hsp70.sev} P{sE-KDN.T} P{sevRas1.V12} P{UAS-Akap200.H} P{UAS-Lk6.H} P{UAS-MESR1.H} P{UAS-MESR6.H} P{UAS-msn.S} P{vg-GAL4.B}
Genes (17)
	Akap200 chb ksr ksr::tor Lk6 MESK1 MESK2 MESK3 MESK4 MESR3 MESR4 MESR6 msn NFAT Ras85D Scer\GAL4 w
Insertions (13)
	P{EP}Akap200^EP2254 P{EP}chb^EP3403 P{EP}Lk6^EP886 P{EP}Lk6^EP3094 P{EP}MESK1^EP980 P{EP}MESK2^EP2347 P{EP}MESK3^EP3728 P{EP}MESK4^EP1015 P{EP}MESR3^EP2221 P{EP}MESR4^EP386 P{EP}MESR6^EP3142 P{EP}msn^EP609 P{EP}NFAT^EP1353