Reference Report

Reference
Citation	Prokopenko, S.N., He, Y., Lu, Y., Bellen, H.J. (2000). Mutations affecting the development of the peripheral nervous system in Drosophila: A molecular screen for novel proteins. Genetics 156(4): 1691--1715. (Export to RIS)
FlyBase ID	FBrf0131381
Publication Type	Research paper
PubMed ID	11102367
PubMed Abstract	In our quest for novel genes required for the development of the embryonic peripheral nervous system (PNS), we have performed three genetic screens using MAb 22C10 as a marker of terminally differentiated neurons. A total of 66 essential genes required for normal PNS development were identified, including 49 novel genes. To obtain information about the molecular nature of these genes, we decided to complement our genetic screens with a molecular screen. From transposon-tagged mutations identified on the basis of their phenotype in the PNS we selected 31 P-element strains representing 26 complementation groups on the second and third chromosomes to clone and sequence the corresponding genes. We used plasmid rescue to isolate and sequence 51 genomic fragments flanking the sites of these P-element insertions. Database searches using sequences derived from the ends of plasmid rescues allowed us to assign genes to one of four classes: (1) previously characterized genes (11), (2) first mutations in cloned genes (1), (3) P-element insertions in genes that were identified, but not characterized molecularly (1), and (4) novel genes (13). Here, we report the cloning, sequence, Northern analysis, and the embryonic expression pattern of candidate cDNAs for 10 genes: astray, chrowded, dalmatian, gluon, hoi-polloi, melted, pebble, skittles, sticky ch1, and vegetable. This study allows us to draw conclusions about the identity of proteins required for the development of the nervous system in Drosophila and provides an example of a molecular approach to characterize en masse transposon-tagged mutations identified in genetic screens.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (6)
	Df(2L)s1402 Df(2R)H3C1 Df(2R)H3D3 Df(2R)H3E1 Df(2R)Np3 Df(2R)stil-A
Alleles (94)
	aay^S042314 abof¹ bon^S024108 btb^k09901 Crc^S114307 CycE⁰⁵²⁰⁶ CycE^k02514 CycE^k05002a dmt^I184 dmt^M572 dmt^S048103 Ecol\lacZ^{Rya-r44F-k00424b} emc^S058701 esg⁰⁵⁷²⁹ esg^k08104 FKBP59^k00424 FKBP59^k09010 fra³ glu^k08819 gui¹ hoip^k07104 insc⁸ insc⁰⁵⁴⁷⁵ insc^k07505 insc^P8 insc^P49 jumu^06142a l(2)k03609^k03609 l(2)k05422a^k05422a l(2)k07127^k07127 l(3)j8B6^j8B6 l(3)S052908a^S052908a lola^5D2 lola^A307 mbl^E27 mbl^E127 mbl^k05507b mbl^k07103 melt^S144114 misn^S081603 pbl⁰⁹⁶⁴⁵ pbl^EP3415 pbl^S008320 pbl^S054203a Pka-C1⁰¹²⁷² ptc⁴ ptc¹⁰ ptc¹¹ ptc¹⁶ ptc^H84 ptc^k02507 puc¹ puc^A251.1F3 puc^H246 puc^S023803 puc^S070806 puc^S135108 RabX1^k06908 rack^k15001 raw⁰⁶⁸²⁵ raw^k01021 raw^k08801 Rya-r44F^k00424b Rya-r44F^k04913 S^k06921 sca¹ Scm^D1 Sema-2a⁰³⁰²¹ Sema-2a^k11240 Sin3A⁰⁸²⁶⁹ Sin3A^ex4 Sin3A^k05415 Sin3A^k09715 Sin3A^k11222 sktl⁰⁵⁴⁷⁵ sktl^k07505 sktl^Δ5 sktl^Δ15 spen¹ stg⁴ stg⁰¹²³⁵ stg^ry80 stg^S024532 stich1^D233 stich1^EP359 stich1^S143702 thr¹ thr^k07805b unch^k15501 veg^k03402 veg^k07202 veg^k07228 veg^Z322 wwy¹
Genes (77)
	aay abof Amph ast barr bon btb bun CG5380 CG8111 CG8281 Crc cwo CycE Cyp6g1 da Dbi Dl dmt E(spl)m8-HLH Ecol\lacZ emc esg FKBP59 fra glu gui h Hey hoip hth hyd insc jumu l(2)35Df l(2)k03609 l(2)k05422a l(2)k07127 l(3)j8B6 l(3)S052908a lola mbl melt misn N numb Oda pav pbl Pka-C1 ptc puc Rab5 RabX1 rack raw RpL30 Rya-r44F S sca Scm Sema-2a sens shn Sin3A sktl SmD3 spdo spen spin stg stich1 thr trbd unch veg wwy
Insertions (47)
	P{EP}EP2116 P{EP}EP2257 P{EP}EP3110 P{EP}melt^EP1019 P{EP}melt^EP3377 P{EP}stich1^EP359 P{lacW}aay^S042314 P{lacW}bon^S024108 P{lacW}btb^k09901 P{lacW}Crc^S114307 P{lacW}CycE^k02514 P{lacW}CycE^k05002a P{lacW}dmt^S048103 P{lacW}emc^S058701 P{lacW}esg^k08104 P{lacW}FKBP59^k00424 P{lacW}glu^k08819 P{lacW}hoip^k07104 P{lacW}insc^k07505 P{lacW}k05422b P{lacW}k06712 P{lacW}k08807 P{lacW}k15501 P{lacW}mbl^k07103 P{lacW}melt^S144114 P{lacW}mi^k06908 P{lacW}pbl^S008320 P{lacW}pbl^S054203a P{lacW}ptc^k02507 P{lacW}puc^S023803 P{lacW}puc^S135108 P{lacW}rack^k15001 P{lacW}raw^k01021 P{lacW}raw^k08801 P{lacW}Rya-r44F^k00424b P{lacW}S081603 P{lacW}S136802 P{lacW}S^k06921 P{lacW}stg^S024532 P{lacW}stich1^S143702 P{lacW}thr^k07805b P{lacW}veg^k03402 P{lacW}veg^k07202 P{lacW}veg^k07228 P{lArB}puc^A251.1F3 P{PZ}insc⁰⁵⁴⁷⁵ P{PZ}jumu^06142a