Reference Report

Reference
Citation	Sotillos, S., Campuzano, S. (2000). DRacGAP, a novel Drosophila gene, inhibits EGFR/Ras signalling in the developing imaginal wing disc. Development 127(24): 5427--5438. (Export to RIS)
FlyBase ID	FBrf0131402
Publication Type	Research paper
PubMed ID	11076763
PubMed Abstract	We have identified a novel Drosophila gene, DRacGAP, which behaves as a negative regulator of Ρ-family GTPases DRac1 and DCdc42. Reduced function of DRacGAP or increased expression of DRac1 in the wing imaginal disc cause similar effects on vein and sensory organ development and cell proliferation. These effects result from enhanced activity of the EGFR/Ras signalling pathway. We find that in the wing disc, DRac1 enhances EGFR/Ras-dependent activation of MAP Kinase in the prospective veins. Interestingly, DRacGAP expression is negatively regulated by the EGFR/Ras pathway in these regions. During vein formation, local DRacGAP repression would ensure maximal activity of Rac and, in turn, of Ras pathways in vein territories. Additionally, maximal expression of DRacGAP at the vein/intervein boundaries would help to refine the width of the veins. Hence, control of DRacGAP expression by the EGFR/Ras pathway is a previously undescribed feedback mechanism modulating the intensity and/or duration of its signalling during Drosophila development.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (2)
	Df(2R)CX1 Df(3R)dsx10M
Alleles (34)
	aos^p1 aos^Scer\UAS.cHa BacA\p35^Scer\UAS.cHa Cdc42³ Cdc42^N17.Scer\UAS Cdc42^V12.Scer\UAS CycE^Scer\UAS.cLa Ecol\lacZ^neur-A101 Ecol\lacZ^{Scer\FRT.Act5C.T:Zzzz\nls1} Hsap\CDKN1A^Scer\UAS.cHa Pak⁴ phl^{K497M.Scer\UAS} Pkn⁰⁶⁷³⁶ Rac1^N17.Scer\UAS Rac1^Scer\UAS.cLa Ras85D^V12.Scer\UAS Rho1^N19.Scer\UAS rho^7M43 rho^ve-1 RhoGEF2⁰⁴²⁹¹ rl^Sem.Scer\UAS Scer\GAL4^69B Scer\GAL4^Bx-MS1096 Scer\GAL4^C-765 Scer\GAL4^dpp.blk1 Scer\GAL4^en-e16E Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} Scer\GAL4^sd-SG29.1 stg^Scer\UAS.cNa tum^{P417Q.Scer\UAS} tum^Scer\UAS.cSa tum^{ΔE1E.Scer\UAS} vn¹ w^+mC
Constructs (19)
	P{Act5C(FRT.polyA)lacZ.nls1} P{GAL4-Act5C(FRT.CD2).P} P{GAL4-dpp.blk1} P{UAS-Cdc42.N17} P{UAS-Cdc42.V12} P{UAS-CycE.L} P{UAS-gil.H} P{UAS-p21.H} P{UAS-p35.H} P{UAS-phl.KMRaf3.1} P{UAS-Rac1.L} P{UAS-Rac1.N17} P{UAS-RacGAP.P417Q} P{UAS-RacGAP.S} P{UAS-RacGAP.ΔE1E} P{UAS-Ras85D.V12} P{UAS-Rho1.N19} P{UAS-Sem} P{UAS-stg.N}
Genes (25)
	aos BacA\p35 cdc2 Cdc42 CycE Ecol\lacZ Egfr Hsap\CDKN1A Pak phl Pkn Rac1 RacGAP84C Ras85D rho Rho1 RhoGEF2 rl rn Scer\GAL4 stg T:Zzzz\nls1 tum vn w
Insertions (8)
	P{en2.4-GAL4}e16E P{GawB}69B P{GawB}Bx^MS1096 P{GawB}C-765 P{GawB}sd^SG29.1 P{lArB}neur^A101 P{PZ}Pkn⁰⁶⁷³⁶ P{PZ}RhoGEF2⁰⁴²⁹¹