|
Reference
|
| Citation |
Horn, C., Jaunich, B., Wimmer, E.A. (2000). Highly sensitive, fluorescent transformation marker for Drosophila transgenesis. Dev. Genes Evol. 210(12): 623--629. |
| FlyBase ID |
FBrf0132292 |
| Type of publication |
Research paper |
| Offprint Available |
Yes |
|
External Crossreferences
|
| PubMed ID |
11151299 |
| PubMed Abstract |
The efficiency of transposon-mediated germline transformation is dependent on the transposon mobility in the host embryo,
and on the detectability of the used transformation marker. Therefore, high susceptibility of the transformation marker to
position effect suppression is a disadvantage. Here we present data that the eye-specific expression of green fluorescent
protein, driven by the 3xP3-EGFP marker, outperforms the commonly used "mini"-white transformation marker in Drosophila germline
transformation experiments: 3xP3-EGFP is more sensitive than "mini"-white in identifying transgenic individuals and reacts
differently to position effect suppression. Therefore, 3xP3-EGFP offers an ideal marker for applications in functional genomics
where as many gene loci as possible should be targeted in the genome of a specific organism, for example, as intended in the
Drosophila gene disruption project. Furthermore, we give a detailed description of the embryonic and larval expression mediated
by the 3xP3-EGFP marker. These pre-adult expression patterns, and the potentially universal applicability of the transformation
marker also offer additional advantages for selecting transgenic individuals in organisms other than Drosophila. This will
be of great interest to the field of evolutionary developmental biology and to modern pest management programs.
|
| Biosis |
2001.28197 |
| Zoological record |
|
Associated Information
|
| Comments |
|
| Text of personal communication |
|
| Associated files |
|
|
Related Publications
|
|
|
|
Also Published As
|
|
|
|
Other Reference Information
|
| Secondary IDs |
|
| Language of publication |
English |
| Additional language(s) of abstract |
|
| ISBN |
|
| Place of publication |
|
|
Published In
|
| Abbreviation |
Dev. Genes Evol. |
| Title |
Development genes and evolution |
| Authors |
|
| Volume range |
206- |
| Year range |
1996- |
| Page range |
|
| Publisher |
|
| Place of publication |
Berlin |
| Language of publication |
English |
| ISBN/ISSN |
0949-944X |
| CODEN |
|
|
Data from Reference
|
| Alleles (3)
|
|
|
| Constructs (22)
|
|
|
| Genes (2)
|
|
|
|
Home