Reference Report

Reference
Citation	Ruhf, M.L., Braun, A., Papoulas, O., Tamkun, J.W., Randsholt, N., Meister, M. (2001). The domino gene of Drosophila encodes novel members of the SWI2/SNF2 family of DNA-dependent ATPases, which contribute to the silencing of homeotic genes. Development 128(8): 1429--1441. (Export to RIS)
FlyBase ID	FBrf0134570
Publication Type	Research paper
PubMed ID	11262242
PubMed Abstract	The Drosophila domino gene has been isolated in a screen for mutations that cause hematopoietic disorders. Generation and analysis of loss-of-function domino alleles show that the phenotypes are typical for proliferation gene mutations. Clonal analysis demonstrates that domino is necessary for cell viability and proliferation, as well as for oogenesis. domino encodes two protein isoforms of 3202 and 2498 amino acids, which contain a common N-terminal region but divergent C termini. The common region includes a 500 amino acid DNA-dependent ATPase domain of the SWI2/SNF2 family of proteins, which function via interaction with chromatin. We show that, although domino alleles do not exhibit homeotic phenotypes by themselves, domino mutations enhance Polycomb group mutations and counteract Trithorax group effects. The Domino proteins are present in large complexes in embryo extracts, and one isoform binds to a number of discrete sites on larval polytene chromosomes. Altogether, the data lead us to propose that domino acts as a repressor by interfering with chromatin structure. This activity is likely to be performed as a subunit of a chromatin-remodeling complex.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (4)
	Df(2L)TE29Aa-11 Df(2R)AA21 Df(2R)Pu-D17 In(1)w^m4h
Alleles (28)
	ash1¹⁷ ash1^B1 brm² dom² dom³ dom⁶ dom⁷ dom⁹ dom¹⁰ dom¹¹ dom¹² dom¹³ dom¹⁴ dom¹⁵ dom¹⁶ dom¹⁷ dom¹⁸ dom^k08108 E(z)⁶⁰ mxc^G43 Pc¹⁶ ph-p⁴¹⁰ Psc¹ Scm^D1 trx^B11 trx^E2 w^+mW.hs w^Fab-7.5F24
Constructs (16)
	P{hsp26-pt-T} P{lacW} P{lacW-Δ2.R} P{lacW-Δ3.R} P{lacW-Δ6.R} P{lacW-Δ7.R} P{lacW-Δ9.R} P{lacW-Δ10.R} P{lacW-Δ11.R} P{lacW-Δ12.R} P{lacW-Δ13.R} P{lacW-Δ15.R} P{lacW-Δ16.R} P{lacW-Δ17.R} P{lacW-Δ18.R} P{UAS-lacZ.Abd-B.5F24}
Genes (14)
	ash1 brm dom E(z) Fab-7 mxc Pc ph-p Psc Scm Scr trx Ubx w
Insertions (15)
	P{hsp26-pt-T}39C-4 P{lacW}dom^k08108 P{lacW-Δ2.R}dom² P{lacW-Δ3.R}dom³ P{lacW-Δ6.R}dom⁶ P{lacW-Δ7.R}dom⁷ P{lacW-Δ9.R}dom⁹ P{lacW-Δ10.R}dom¹⁰ P{lacW-Δ11.R}dom¹¹ P{lacW-Δ12.R}dom¹² P{lacW-Δ13.R}dom¹³ P{lacW-Δ15.R}dom¹⁵ P{lacW-Δ16.R}dom¹⁶ P{lacW-Δ17.R}dom¹⁷ P{lacW-Δ18.R}dom¹⁸