Reference Report

Reference
Citation	Plaza, S., Prince, F., Jaeger, J., Kloter, U., Flister, S., Benassayag, C., Cribbs, D., Gehring, W.J. (2001). Molecular basis for the inhibition of Drosophila eye development by Antennapedia. EMBO J. 20(4): 802--811. (Export to RIS)
FlyBase ID	FBrf0134592
Publication Type	Research paper
PubMed ID	11179224
PubMed Abstract	Hox genes encoding homeodomain transcriptional regulators are known to specify the body plan of multicellular organisms and are able to induce body plan transformations when misexpressed. These findings led to the hypothesis that duplication events and misexpression of Hox genes during evolution have been necessary for generating the observed morphological diversity found in metazoans. It is known that overexpressing Antennapedia (Antp) in the head induces antenna-to-leg as well as head-to-thorax transformation and eye reduction. At present, little is known about the exact molecular mechanism causing these phenotypes. The aim of this study is to understand the basis of inhibition of eye development. We demonstrate that Antp represses the activity of the eye regulatory cascade. By ectopic expression, we show that Antp antagonizes the activity of the eye selector gene eyeless. Using both in vitro and in vivo experiments, we demonstrate that this inhibitory mechanism involves direct protein-protein interactions between the DNA-binding domains of EY and ANTP, resulting in mutual inhibition.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Alleles (20)
	abd-A^Scer\UAS.cPa Abd-B^Scer\UAS.cCa Antp^{A50,51.Scer\UAS} Antp^G2 Antp^G8 Antp^G10 Antp^G11 Antp^K50.Scer\UAS Antp^Scer\UAS.cBa Antp^{ΔHD.Scer\UAS} Ecol\lacZ^so-7 Ecol\lacZ^so.10 ey^hs.PH ey^Scer\UAS.cHa Scer\GAL4^dpp.blk1 Scer\GAL4^ey.PH Scer\GAL4^ey-OK107 Scer\GAL4^GMR.PF Scr^Scer\UAS.cPa Ubx^Scer\UAS.cPa
Constructs (13)
	P{GAL4-dpp.blk1} P{GAL4-ey.H} P{GAL4-ninaE.GMR} P{hs-ey} P{so-lacZ.10} P{UAS-abd-A.P} P{UAS-Antp.A50,51} P{UAS-Antp.B} P{UAS-Antp.Q50K.C} P{UAS-AntpΔHD} P{UAS-ey.H} P{UAS-Scr.P} P{UAS-Ubx.P}
Genes (15)
	abd-A Abd-B Antp dac Ecol\lacZ ey eya eyg futsch Optix Scer\GAL4 Scr so toy Ubx
Insertions (2)
	P{A92}so⁷ P{GawB}ey^OK107