Reference Report

Reference
Citation	Zervas, C.G., Gregory, S.L., Brown, N.H. (2001). Drosophila integrin-linked kinase is required at sites of integrin adhesion to link the cytoskeleton to the plasma membrane. J. Cell Biol. 152(5): 1007--1018. (Export to RIS)
FlyBase ID	FBrf0134704
Publication Type	Research paper
PubMed ID	11238456
PubMed Abstract	Integrin-linked kinase (ILK) was identified by its interaction with the cytoplasmic tail of human beta1 integrin and previous data suggest that ILK is a component of diverse signaling pathways, including integrin, Wnt, and protein kinase B. Here we show that the absence of ILK function in Drosophila causes defects similar to loss of integrin adhesion, but not similar to loss of these signaling pathways. ILK mutations cause embryonic lethality and defects in muscle attachment, and clones of cells lacking ILK in the adult wing fail to adhere, forming wing blisters. Consistent with this, an ILK-green fluorescent protein fusion protein colocalizes with the position-specific integrins at sites of integrin function: muscle attachment sites and the basal junctions of the wing epithelium. Surprisingly, mutations in the kinase domain shown to inactivate the kinase activity of human ILK do not show any phenotype in Drosophila, suggesting a kinase-independent function for ILK. The muscle detachment in ILK mutants is associated with detachment of the actin filaments from the muscle ends, unlike integrin mutants, in which the primary defect is detachment of the plasma membrane from the extracellular matrix. Our data suggest that ILK is a component of the structure linking the cytoskeleton and the plasma membrane at sites of integrin-mediated adhesion.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (3)
	Df(3L)ME107 Df(3L)Pc-14d T(2;3)Ilk²
Alleles (21)
	arm⁴ Avic\GFP^{S65T.I167T.Scer\UAS.T:Myr-Src64B} Ecol\lacZ^PY258 if^B4 Ilk¹ Ilk² Ilk^+t9 Ilk^{E359K.Scer\UAS} Ilk^{E359K.T:Avic\GFP-m6} Ilk^{K219M.T:Avic\GFP-m6} Ilk^{P358S.T:Avic\GFP-m6} Ilk^Scer\UAS.cZa Ilk^{T:Avic\GFP-m6} mys¹¹ Scer\GAL4^48Y Scer\GAL4^69B Scer\GAL4^arm.PS Scer\GAL4^en-e16E Scer\GAL4^how-24B Scer\GAL4^twi.PG T:Avic\GFP^m6
Constructs (10)
	P{GAL4-arm.S} P{GAL4-twi.G} P{Ilk^+t9} P{Ilk^{E359K.T:Avic\GFP-m6}} P{Ilk^{K219M.T:Avic\GFP-m6}} P{Ilk^{P358S.T:Avic\GFP-m6}} P{Ilk^{T:Avic\GFP-m6}} P{UAS-Ilk.E359K} P{UAS-Ilk.Z} P{UAS-Src-GFP(S65T/I167T)}
Genes (9)
	arm Avic\GFP Ecol\lacZ if Ilk mys Scer\GAL4 T:Avic\GFP T:Myr-Src64B
Insertions (5)
	P{en2.4-GAL4}e16E P{GawB}48Y P{GawB}69B P{GawB}how^24B P{PZ}PY258