Park, J.M., Gim, B.S., Kim, J.M., Yoon, J.H., Kim, H.S., Kang, J.G., Kim, Y.J. (2001). Drosophila mediator complex is broadly utilized by diverse gene-specific transcription factors at different types of core promoters.  Mol. Cell. Biol. 21(7): 2312--2323.

FBrf0134769

Research paper

To decipher the mechanistic roles of Mediator proteins in regulating developmental specific gene expression and compare them to those of TATA-binding protein (TBP)-associated factors (TAFs), we isolated and analyzed a multiprotein complex containing Drosophila Mediator (dMediator) homologs. dMediator interacts with several sequence-specific transcription factors and basal transcription machinery and is critical for activated transcription in response to diverse transcriptional activators. The requirement for dMediator did not depend on a specific core promoter organization. By contrast, TAFs are preferentially utilized by promoters having a specific core element organization. Therefore, Mediator proteins are suggested to act as a pivotal coactivator that integrates promoter-specific activation signals to the basal transcription machinery.

PMC86865 (PMC) (EuropePMC)