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Reference
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| Citation |
Eeken, J.C.J., Romeijn, R.J., de Jong, A.W.M., Pastink, A., Lohman, P.H.M. (2001). Isolation and genetic characterisation of the Drosophila homologue of (SCE)REV3, encoding the catalytic subunit of DNA polymerase
. Mutat. Res. 485(3): 237--253. |
| FlyBase ID |
FBrf0134782 |
| Type of publication |
Research paper |
| Offprint Available |
Yes |
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External Crossreferences
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| PubMed ID |
11267835 |
| PubMed Abstract |
In Drosophila, about 30 mutants are known that show hypersensitivity to the methylating agent methyl methane sulfonate (MMS).
Addition of this agent to the medium results in an increased larval mortality of the mutants. Using a P-insertion mutagenesis
screen, three MMS-sensitive mutants on chromosome II were isolated. One of these is allelic to the known EMS-induced mus205
(mutagen sensitive) mutant. In the newly isolated mutant, a P-element is detected in region 43E by in situ hybridisation.
The localisation of mus205 to this region was confirmed by deficiency mapping. The gene was cloned and shows strong homology
to the Saccharomyces cerevisiae REV3 gene. The REV3 gene encodes the catalytic subunit of DNA polymerase zeta, involved in
translesion synthesis. The P-element is inserted in the first exon of the mus205 gene resulting in an aberrant mRNA, encoding
a putative truncated protein containing only the first 13 of the 2130 aa native Drosophila protein. The mus205 mutant is hypersensitive
to alkylating agents and UV, but not to ionising radiation. In contrast to reported data, in germ cells, the mutant has no
effect on mutability by X-rays, NQO and alkylating agents. In somatic cells, the mutant shows no effect on MMS-induced mutations
and recombinations. This phenotype of the Drosophila mus205 mutant is strikingly different from the phenotype of the yeast
rev3 mutant, which is hypomutable after UV, X-rays, NQO and alkylating agents.
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| Biosis |
2001.209710 |
| Zoological record |
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Associated Information
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| Comments |
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| Text of personal communication |
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Related Publications
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
Mutat. Res. |
| Title |
Mutation Research |
| Authors |
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| Volume range |
1- |
| Year range |
1964- |
| Page range |
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| Publisher |
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| Place of publication |
Amsterdam |
| Language of publication |
English |
| ISBN/ISSN |
0027-5107 |
| CODEN |
MUREAV |
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Data from Reference
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| Aberrations (3)
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| Alleles (7)
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| Genes (20)
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| Insertions (7)
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