Reference Report

Reference
Citation	Eeken, J.C.J., Romeijn, R.J., de Jong, A.W.M., Pastink, A., Lohman, P.H.M. (2001). Isolation and genetic characterisation of the Drosophila homologue of (SCE)REV3, encoding the catalytic subunit of DNA polymerase . Mutat. Res. 485(3): 237--253. (Export to RIS)
FlyBase ID	FBrf0134782
Publication Type	Research paper
PubMed ID	11267835
PubMed Abstract	In Drosophila, about 30 mutants are known that show hypersensitivity to the methylating agent methyl methane sulfonate (MMS). Addition of this agent to the medium results in an increased larval mortality of the mutants. Using a P-insertion mutagenesis screen, three MMS-sensitive mutants on chromosome II were isolated. One of these is allelic to the known EMS-induced mus205 (mutagen sensitive) mutant. In the newly isolated mutant, a P-element is detected in region 43E by in situ hybridisation. The localisation of mus205 to this region was confirmed by deficiency mapping. The gene was cloned and shows strong homology to the Saccharomyces cerevisiae REV3 gene. The REV3 gene encodes the catalytic subunit of DNA polymerase zeta, involved in translesion synthesis. The P-element is inserted in the first exon of the mus205 gene resulting in an aberrant mRNA, encoding a putative truncated protein containing only the first 13 of the 2130 aa native Drosophila protein. The mus205 mutant is hypersensitive to alkylating agents and UV, but not to ionising radiation. In contrast to reported data, in germ cells, the mutant has no effect on mutability by X-rays, NQO and alkylating agents. In somatic cells, the mutant shows no effect on MMS-induced mutations and recombinations. This phenotype of the Drosophila mus205 mutant is strikingly different from the phenotype of the yeast rev3 mutant, which is hypomutable after UV, X-rays, NQO and alkylating agents.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mutat. Res.
Title	Mutation Research
Publication Year	1964-
ISBN/ISSN	0027-5107
Data from Reference
Aberrations (3)
	Df(2R)NCX8 Df(2R)NCX13 Df(2R)ST1
Alleles (7)
	MMS-1^MMS-1 MMS-2^MMS-2 MMS-3^MMS-3 MMS-4^MMS-4 MMS-6^MMS-6 mus201^MMS-5 mus205^P
Genes (20)
	blow boca CanB2 cn Egfr fs(2)43Ei Gapdh1 l(2)43Ef l(2)43Eg l(2)43Eh MMS-1 MMS-2 MMS-3 MMS-4 MMS-6 mus201 mus205 Rpt1 scra U2A
Insertions (7)
	P{}MMS-1^MMS-1 P{}MMS-2^MMS-2 P{}MMS-3^MMS-3 P{}MMS-4^MMS-4 P{}MMS-6^MMS-6 P{}mus201^MMS-5 P{}mus205^P