Reference Report

Reference
Citation	Sonoda, J., Wharton, R.P. (2001). Drosophila Brain Tumor is a translational repressor. Genes Dev. 15(6): 762--773. (Export to RIS)
FlyBase ID	FBrf0135777
Publication Type	Research paper
PubMed ID	11274060
PubMed Abstract	The Drosophila brain tumor (brat) gene encodes a member of the conserved NHL family of proteins, which appear to regulate differentiation and growth in a variety of organisms. One of the founding family members, Caenorhabditis elegans LIN-41, is thought to control posttranscriptional gene expression. However, the mechanism by which LIN-41, or any other NHL protein, acts has not been clear. Using a yeast "four-hybrid" interaction assay, we show that Brain Tumor is recruited to hunchback (hb) mRNA through interactions with Nanos and Pumilio, which bind to the RNA to repress its translation. Interaction with the Nanos/Pumilio/RNA complex is mediated by the Brat NHL domain; single amino acid substitutions in this domain compromise quaternary complex assembly in vitro and hb regulation in vivo. Thus, recruitment of Brat is necessary for translational repression and the normal development of posterior embryonic pattern. In addition to regulating abdominal segmentation, previous genetic analysis has shown that Brat, Nanos, and Pumilio govern a variety of developmental processes. We examined the role of Brat in two of these processes-regulation of maternal Cyclin B mRNA in the embryo and regulation of imaginal disc development. The results of these experiments suggest that NHL domain proteins are recruited to various mRNAs by combinatorial protein-protein interactions.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Aberrations (2)
	Df(2L)TE37C-7 Df(3R)Dl-FX1
Alleles (27)
	brat¹ brat^fs1.Scer\UAS brat^fs1 brat^fs3 brat^k06028 brat^Scer\UAS.cSa brat^{Δ63-721.fs1.Scer\UAS} brat^{Δ63-721.Scer\UAS} brat^{Δ724-1037.Scer\UAS} nos⁰⁷¹¹⁷ nos^BN nos^j3B6 pum¹³ pum^FC8 pum^Msc pum^Δ2'.C1365R pum^Δ2'.G1186D pum^Δ2'.G1222E pum^Δ2'.G1285D pum^Δ2'.G1330D pum^Δ2'.G1330Y pum^Δ2'.K1331Y pum^Δ2'.nos pum^Δ2'.T1366D Scer\GAL4^Act5C.PI Scer\GAL4^en-e16E Scer\GAL4^{nos.UTR.T:Hsim\VP16}
Constructs (15)
	P{Act5C-GAL4} P{GAL4::VP16-nos.UTR} P{nos-pum.Δ2'.C1365R} P{nos-pum.Δ2'.G1186D} P{nos-pum.Δ2'.G1222E} P{nos-pum.Δ2'.G1285D} P{nos-pum.Δ2'.G1330D} P{nos-pum.Δ2'.G1330Y} P{nos-pum.Δ2'.K1331Y} P{nos-pum.Δ2'.T1366D} P{UAS-brat.fs1.Δ63-721} P{UAS-brat.fs1} P{UAS-brat.S} P{UAS-brat.Δ63-721} P{UAS-brat.Δ724-1037}
Genes (6)
	brat CycB nos pum Scer\GAL4 T:Hsim\VP16
Insertions (1)
	P{en2.4-GAL4}e16E