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Reference Report
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| Citation | Donaldson, M.M., Tavares, A.A.A., Ohkura, H., Deak, P., Glover, D.M. (2001). Metaphase arrest with centromere separation in polo mutants of Drosophila. J. Cell Biol. 153(4): 663--676. (Export to RIS) | ||
| FlyBase ID | FBrf0135842 | ||
| Publication Type | Research paper | ||
| External Crossreferences | |||
| PubMed ID | 11352929 | ||
| PubMed Abstract | The Drosophila gene polo encodes a conserved protein kinase known to be required to organize spindle poles and for cytokinesis. Here we report two strongly hypomorphic mutations of polo that arrest cells of the larval brain at a point in metaphase when the majority of sister kinetochores have separated by between 20-50% of the total spindle length in intact cells. In contrast, analysis of sister chromatid separation in squashed preparations of cells indicates that some 83% of sisters remain attached. This suggests the separation seen in intact cells requires the tension produced by a functional spindle. The point of arrest corresponds to the spindle integrity checkpoint; Bub1 protein and the 3F3/2 epitope are present on the separated kinetochores and the arrest is suppressed by a bub1 mutation. The mutant mitotic spindles are anastral and have assembled upon centrosomes that are associated with Centrosomin and the abnormal spindle protein (Asp), but neither with gamma-tubulin nor CP190. We discuss roles for Polo kinase in recruiting centrosomal proteins and in regulating progression through the metaphase-anaphase checkpoint. | ||
| BIOSIS ID | 2001.277436 | ||
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| Language of Publication | English | ||
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Parent Publication
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| Publication Type | Journal | ||
| Abbreviation | J. Cell Biol. | ||
| Title | Journal of Cell Biology | ||
| Editors | |||
| Publication Year | 1966- | ||
| ISBN/ISSN | 0021-9525 | ||
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Data from Reference
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| Alleles (5)
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| Genes (14)
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| Insertions (2)
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