HomeReference Report
| Reference | |
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| Citation | Luo, L. (2001.5.2). Luo constructs and insertions. |
| FlyBase ID | FBrf0136038 |
| Type of publication | Personal communication to FlyBase |
| Offprint Available | No |
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| Text of personal communication |
From kcook@XXXXXXXXXXXXXXX Wed May 02 15:42:51 2001
To: flybase-updates@XXXXXXXXXXXXXXX From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> Subject: Luo constructs and insertions The following information accompanied stocks donated to the Bloomington Stock Center by Liqun Luo, Stanford University (5/01). In the entry for P{UAS-Cdc42.S89} (FBtp0008218), two mutant Cdc42 constructs are described as synonymous, an 'S89' construct and an 'L89' construct. According to Liqun Luo: >'These are in fact two different transgenes. The wild-type amino acid at >89 position is Ser. Ser to Phe change (F89) was trying to mimic a >dominant negative mutant in worm ras. L89 (Ser changes to Leu) was an >error in making that intended F89 mutation. However we later found that >L89 and F89 did have similar phenotypes when both transgenes are compared >side-by-side. According to the convention S89 should be renamed F89. So >we should keep L89 as it is and change S89 to F89.' Insertions of these and other constructs from Luo include: P{UAS-Cdc42.F89}3 third chromosome insertion P{UAS-Cdc42.V12}2 third chromosome insertion P{UAS-Cdc42.N17}3 second chromosome insertion P{UAS-Cdc42.L89}4 third chromosome insertion P{UAS-Drac1.L89}6 second chromosome insertion P{UAS-Drac1.V12}1 third chromosome insertion P{UAS-Drac1.N17}1 third chromosome insertion P{UAS-Drac1.L}3 second chromosome insertion All are homozygous viable and fertile insertions. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-855-5782 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-3700 kcook@XXXXXXXXXXXXXXX |
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| Language of publication | English |
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| Language of publication | English |
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Data from Reference
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| Alleles (2)
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| Constructs (2)
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| Genes (1)
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| Insertions (8)
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