Reference Report

Reference
Citation	Fazio, I.K., Bolger, T.A., Gill, G. (2001). Conserved regions of the Drosophila erect wing protein contribute both positively and negatively to transcriptional activity. J. Biol. Chem. 276(22): 18710--18716. (Export to RIS)
FlyBase ID	FBrf0136742
Publication Type	Research paper
PubMed ID	11278998
PubMed Abstract	Genetic studies of the Drosophila erect wing (ewg) gene have revealed that ewg has an essential function in the embryonic nervous system and is required for the specification of certain muscle cells. We have found that EWG is a site-specific transcriptional activator, and we report here that evolutionarily conserved regions of EWG contribute both positively and negatively to transcriptional activity. Using gel mobility shift assays, we have shown that an EWG dimer binds specifically to DNA. In transfection assays, EWG activated expression of a reporter gene bearing specific binding sites. Analysis of deletion mutants and fusions of EWG to the Gal4 DNA binding domain has identified a transcriptional activation domain in the C terminus of EWG. Deletion analysis also revealed a novel inhibitory region in the N terminus of EWG. Strikingly, both the activation domain and the inhibitory region are conserved in EWG homologs including human nuclear respiratory factor 1 (NRF-1) and the sea urchin P3A2 protein. The strong conservation of elements that determine transcriptional activity suggests that the EWG, NRF-1, and P3A2 family of proteins shares common mechanisms of action and has maintained common functions across evolution.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Biol. Chem.
Title	Journal of Biological Chemistry
Publication Year	1905-
ISBN/ISSN	0021-9258
Data from Reference
Alleles (35)
	ewg^{1-144.Act5C.T:Scer\GAL4} ewg^350.Act5C ewg^564.Act5C ewg^631.Act5C ewg^654.Act5C ewg^Act5C.PF ewg^{Act5C.T:Zzzz\FLAG} ewg^{C3.Act5C.T:Scer\GAL4} ewg^{C4.Act5C.T:Scer\GAL4} ewg^{C5.Act5C.T:Scer\GAL4} ewg^{fAD.Act5C.T:Scer\GAL4} ewg^{M1.Act5C.T:Scer\GAL4} ewg^{M2.Act5C.T:Scer\GAL4} ewg^{M3.Act5C.T:Scer\GAL4} ewg^{M3IV.Act5C.T:Scer\GAL4} ewg^{M3SY.Act5C.T:Scer\GAL4} ewg^{M3TT.Act5C.T:Scer\GAL4} ewg^{M3VV.Act5C.T:Scer\GAL4} ewg^{M4.Act5C.T:Scer\GAL4} ewg^{M5.Act5C.T:Scer\GAL4} ewg^{M6.Act5C.T:Scer\GAL4} ewg^{N1.Act5C.T:Scer\GAL4} ewg^{N2.Act5C.T:Scer\GAL4} ewg^{N3.Act5C.T:Scer\GAL4} ewg^{N4.Act5C.T:Scer\GAL4} ewg^{N5.Act5C.T:Scer\GAL4} ewg^{NΔ86.Act5C.T:Zzzz\FLAG} ewg^{NΔ144.Act5C.T:Zzzz\FLAG} ewg^{Δ87-130.Act5C.T:Zzzz\FLAG} ewg^{Δ87-144.Act5C.T:Zzzz\FLAG} ewg^{Δ130-144.Act5C.T:Zzzz\FLAG} Ppyr\LUC^E1B Ppyr\LUC^ewg.BS3 Ppyr\LUC^Scer\UAS.G5 Scer\GAL4^1-147.Act5C
Genes (5)
	ewg Ppyr\LUC Scer\GAL4 T:Scer\GAL4 T:Zzzz\FLAG