Reference Report

Reference
Citation	LaJeunesse, D.R., McCartney, B.M., Fehon, R.G. (2001). A Systematic screen for dominant second-site modifiers of Merlin/NF2 Phenotypes reveals an interaction with blistered/DSRF and scribbler. Genetics 158(2): 667--679. (Export to RIS)
FlyBase ID	FBrf0136951
Publication Type	Research paper
PubMed ID	11404331
PubMed Abstract	Merlin, the Drosophila homologue of the human tumor suppressor gene Neurofibromatosis 2 (NF2), is required for the regulation of cell proliferation and differentiation. To better understand the cellular functions of the NF2 gene product, Merlin, recent work has concentrated on identifying proteins with which it interacts either physically or functionally. In this article, we describe genetic screens designed to isolate second-site modifiers of Merlin phenotypes from which we have identified five multiallelic complementation groups that modify both loss-of-function and dominant-negative Merlin phenotypes. Three of these groups, Group IIa/scribbler (also known as brakeless), Group IIc/blistered, and Group IId/net, are known genes, while two appear to be novel. In addition, two genes, Group IIa/scribbler and Group IIc/blistered, alter Merlin subcellular localization in epithelial and neuronal tissues, suggesting that they regulate Merlin trafficking or function. Furthermore, we show that mutations in scribbler and blistered display second-site noncomplementation with one another. These results suggest that Merlin, blistered, and scribbler function together in a common pathway to regulate Drosophila wing epithelial development.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (38)
	Df(2L)30A-C Df(2L)al Df(2L)ast2 Df(2L)C144 Df(2L)cact-255rv64 Df(2L)cl-h3 Df(2L)H20 Df(2L)J39 Df(2L)JS32 Df(2L)Mdh Df(2L)net62 Df(2L)net¹⁰⁷ Df(2L)net-PM47C Df(2L)net-PMF Df(2R)44CE Df(2R)cn88b Df(2R)en30 Df(2R)en-A Df(2R)M41A Df(2R)nap1 Df(2R)or-BR6 Df(2R)PC4 Df(2R)pk78s Df(2R)Px2 Df(3L)AC1 Df(3L)brm11 Df(3L)HR119 Df(3L)VW3 Df(3L)ZN47 Df(3R)Antp17 Df(3R)awd-KRB Df(3R)Cha7 Df(3R)e-N19 Df(3R)P14 Df(3R)ry615 Df(3R)T-32 Df(3R)Tl-P In(2R)bw^VDe2LCy^R
Alleles (34)
	bs²¹¹ bs²¹⁷ bs²²¹ bs²³⁷ bs²⁴² bs²⁴⁶ bs²⁵³ bs³⁶⁴ bs⁰³²⁶⁷ bs^unspecified E(Mer)IIb¹⁸² E(Mer)IIb¹⁸⁷ E(Mer)IIb²⁰⁹ E(Mer)IIIa²⁰² E(Mer)IIIa²³⁹ E(Mer)IIIa²⁷⁸ E(Mer)IIIa³²⁰ Mer³ Mer^{ΔBB.Scer\UAS.T:Hsap\MYC} net¹⁰⁷ net³⁸³ sbb⁴ sbb⁹⁴ sbb¹⁵¹ sbb²¹⁶ sbb²⁵⁶ sbb²⁵⁹ sbb²⁷⁰ sbb³²⁴ sbb⁰⁴⁴⁴⁰ sbb^k00702 sbb^unspecified Scer\GAL4^ap-md52 Scer\GAL4^en-e16E
Constructs (1)
	P{UAS-Mer^ΔBB.myc}
Genes (16)
	ap arm bhe bs cora E(Mer)IIb E(Mer)IIIa l(2)gl Mer Moe N net sbb Scer\GAL4 T:Hsap\MYC α-Spec
Insertions (6)
	P{GAL4}md52 P{GawB}ptc^559.1 P{lacW}ban^L1170a P{lacW}l(3)j2B9^j2B9 P{lacW}lid^k06801 P{lacW}Pen^k14401a