Reference Report

Reference
Citation	Faucheux, M., Netter, S., Bloyer, S., Moussa, M., Boissonneau, E., Lemeunier, F., Wegnez, M., Theodore, L. (2001). Advantages of a P-element construct containing MtnA sequences for the identification of patterning and cell determination genes in Drosophila melanogaster. Mol. Genet. Genomics 265(1): 14--22. (Export to RIS)
FlyBase ID	FBrf0137022
Publication Type	Research paper
PubMed ID	11370860
PubMed Abstract	The P[MTW] transposon carries a functional MtnA (metallothionein) gene and a miniwhite reporter gene. When P[MTW] was transformed into Drosophila, many lines were found to show position-dependent expression patterns of the miniwhite or the MtnA transgene. Identification of several of the target genes indicated that this construct behaves as an enhancer or silencer trap. For instance, expression of at least one reporter transgene was shown to correlate with that of the endogenous gene in the case of insertions in Ultrabithorax, four-jointed, and the iroquois complex. The frequency of patterns recovered with P[MTW] is higher than that reported for P[LacW], suggesting that P[MTW] has unusual properties. The possibility of biased insertion of P[MTW] was assayed by screening a sample of 66 MTW lines for modifiers of the extra sex comb phenotype caused by a hypomorphic allele of polyhomeotic. Seven modifiers were recovered, which could be ranked in two classes: genes involved in leg morphogenesis (including four-jointed and spitz), and genes of the Polycomb- or trithorax-Group, including trithorax and batman, a new gene which encodes a product with a BTB/POZ domain. Taken together, these results indicate that P[MTW] allows the tagging of patterning and cell determination genes, and thus provides a useful tool for identifying new developmental functions.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Genet. Genomics
Title	Molecular Genetics and Genomics
Publication Year	2001-
ISBN/ISSN	1617-4615
Data from Reference
Aberrations (2)
	Df(2L)TW50 Df(2R)lolal^311a
Alleles (17)
	caup^L daw^313.15 dpa^311.5 Eip75B^333.15 fj^333a lolal^311a lolal^k02512 MtnA¹ MtnA³ MtnA^MTW Pc¹⁶ ph-p⁴¹⁰ spi^133a spi^SC1 trx^311.28 Ubx^111c w^+mC
Constructs (1)
	P{MTW}
Genes (19)
	ara caup daw dpa Eip75B emc fj KdelR lolal MtnA neur Pc Pcl ph-p spi trx Ubx w wb
Insertions (21)
	P{MTW}111d P{MTW}133c P{MTW}133e P{MTW}133f P{MTW}311a P{MTW}311b P{MTW}313a P{MTW}313c P{MTW}313d P{MTW}313e P{MTW}313f P{MTW}B P{MTW}C P{MTW}caup^L P{MTW}daw^313.15 P{MTW}dpa^311.5 P{MTW}Eip75B^333.15 P{MTW}fj^333a P{MTW}spi^133a P{MTW}trx^311.28 P{MTW}Ubx^111c